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03001nam a22002895i 4500 |
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978-1-59259-517-4 |
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20190618092058.0 |
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cr nn 008mamaa |
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100301s1994 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592595174
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| 024 |
7 |
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|a 10.1385/0896032566
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 100 |
1 |
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|a Kneale, G. Geoff.
|e author.
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| 245 |
1 |
0 |
|a DNA'Protein Interactions
|b Principles and Protocols /
|c by G. Geoff Kneale.
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| 250 |
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|a 1st ed. 1994.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1994.
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| 300 |
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|a XV, 427 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 30
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| 505 |
0 |
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|a DNase I Footprinting -- Footprinting with Exonuclease III -- Hydroxyl Radical Footprinting -- Hydroxyl Radical Interference -- 1,10-Phenanthroline-Copper Ion Nuclease Footprinting of DNA-Protein Complexes in Situ Following Mobility-Shift Electrophoresis Assays -- Identification of Protein-DNA Contacts with Dimethyl Sulfate -- Diethyl Pyrocarbonate as a Probe of Protein-DNA Interactions -- Osmium Tetroxide Modification and the Study of DNA-Protein Interactions -- Diffusible Singlet Oxygen as a Probe of DNA Deformation -- Ethylation Interference -- Uranyl Photofootprinting of DNA-Protein Complexes -- Nitration of Tyrosine Residues in Protein-Nucleic Acid Complexes -- Limited Proteolysis of Protein-Nucleic Acid Complexes -- Cloning and Expression of DNA Binding Domains Using PCR -- Overexpression and Purification of Eukaryotic Transcription Factors as Glutathione-S-Transferase Fusions in E. coli -- Site-Directed Mutagenesis by the Cassette Method -- Site-Directed and Site-Saturation Mutagenesis Using Oligonucleotide Primers -- UV Laser-Induced Protein-DNA Crosslinking -- Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine -- Filter-Binding Assays -- The Gel Shift Assay for the Analysis of DNA-Protein Interactions -- Improved Plasmid Vectors for the Analysis of Protein-Induced DNA Bending -- Determination of Sequence Preferences of DNA Binding Proteins Using Pooled Solid-Phase Sequencing of Low Degeneracy Oligonucleotide Mixtures -- Analysis of DNA-Protein Interactions by Intrinsic Fluorescence -- A Competition Assay for DNA Binding Using the Fluorescent Probe ANS -- Circular Dichroism for the Analysis of Protein-DNA Interactions -- Electron Microscopy of Protein-Nucleic Acid Complexes -- Reconstitution of Protein-DNA Complexes for Crystallization -- Assay of Restriction Endonucleases Using Oligonucleotides -- Assays for Restriction Endonucleases Using Plasmid Substrates -- Assays for Transcription Factor Activity -- An Assay for In Vitro Recombination Between Duplex DNA Molecules.
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| 650 |
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|a Biochemistry.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1385/0896032566
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