DNA'Protein Interactions Principles and Protocols /
| Autor principal: | |
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| Autor Corporativo: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1994.
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| Edición: | 1st ed. 1994. |
| Colección: | Methods in Molecular Biology,
30 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/0896032566 |
Tabla de Contenidos:
- DNase I Footprinting
- Footprinting with Exonuclease III
- Hydroxyl Radical Footprinting
- Hydroxyl Radical Interference
- 1,10-Phenanthroline-Copper Ion Nuclease Footprinting of DNA-Protein Complexes in Situ Following Mobility-Shift Electrophoresis Assays
- Identification of Protein-DNA Contacts with Dimethyl Sulfate
- Diethyl Pyrocarbonate as a Probe of Protein-DNA Interactions
- Osmium Tetroxide Modification and the Study of DNA-Protein Interactions
- Diffusible Singlet Oxygen as a Probe of DNA Deformation
- Ethylation Interference
- Uranyl Photofootprinting of DNA-Protein Complexes
- Nitration of Tyrosine Residues in Protein-Nucleic Acid Complexes
- Limited Proteolysis of Protein-Nucleic Acid Complexes
- Cloning and Expression of DNA Binding Domains Using PCR
- Overexpression and Purification of Eukaryotic Transcription Factors as Glutathione-S-Transferase Fusions in E. coli
- Site-Directed Mutagenesis by the Cassette Method
- Site-Directed and Site-Saturation Mutagenesis Using Oligonucleotide Primers
- UV Laser-Induced Protein-DNA Crosslinking
- Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine
- Filter-Binding Assays
- The Gel Shift Assay for the Analysis of DNA-Protein Interactions
- Improved Plasmid Vectors for the Analysis of Protein-Induced DNA Bending
- Determination of Sequence Preferences of DNA Binding Proteins Using Pooled Solid-Phase Sequencing of Low Degeneracy Oligonucleotide Mixtures
- Analysis of DNA-Protein Interactions by Intrinsic Fluorescence
- A Competition Assay for DNA Binding Using the Fluorescent Probe ANS
- Circular Dichroism for the Analysis of Protein-DNA Interactions
- Electron Microscopy of Protein-Nucleic Acid Complexes
- Reconstitution of Protein-DNA Complexes for Crystallization
- Assay of Restriction Endonucleases Using Oligonucleotides
- Assays for Restriction Endonucleases Using Plasmid Substrates
- Assays for Transcription Factor Activity
- An Assay for In Vitro Recombination Between Duplex DNA Molecules.