In Vitro Transcription and Translation Protocols

In Vitro Transcription and Translation Protocols

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Tymms, Martin J. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 1995.
Edition:1st ed. 1995.
Series:Methods in Molecular Biology, 37
Subjects:
Cytology.
Cell Biology.
Online Access:https://doi.org/10.1385/0896032884
Table of Contents:
  • Transcription In Vitro Using Bacteriophage RNA Polymerases
  • Subtraction Hybridization cDNA Libraries
  • Quantitative Measurement of mRNA Using the RNase Protection Assay
  • Assembly and Transcription of Chromatin Templates Using RNA Polymerase III
  • Antisense Affinity Depletion of RNP Particles
  • In Vitro mRNA Editing Using S100 Extracts
  • An In Vitro Transcription Assay for Probing Drug-DNA Interactions During Active Transcription of DNA
  • In Vitro Reconstitution of Progesterone-Dependent RNA Transcription in Nuclear Extracts of Human Breast Carcinoma Cells
  • Plastid In Vitro Transcription
  • Microinjection of In Vitro Transcribed RNA and Antisense Oligonucleotides in Mouse Oocytes and Early Embryos to Study the Gain-and Loss-of-Function of Genes
  • mRNA Translation in Xenopus Oocytes
  • Transcription System Using a HeLa Cell Mitochondrial Lysate
  • The Xenopus Egg Extract Translation System
  • In Vitro Translation Using Rabbit Reticulocyte Lysate
  • An In Vitro Transcription System from BmN Cells of the Silkworm, Bombyx mori
  • Translation Using a Wheat-Germ Extract
  • In Vitro Transcription and Translation in a Cell-Free System from Clostridium tetani
  • Preparation and Use of E. coli S-30 Extracts
  • Posttranslational Transport and In Thylakoido Integration of Plastid Protein
  • Import into isolated yeast mitochondria of radiolabeled proteins synthesized in vitro
  • Structure-Function studies based on in vitro expression
  • ?-Like Plastid Transcription Factors
  • Preparation of HeLa Nuclear Extracts
  • Purification of the human TATA-Binding Protein, TBP
  • DNA-Binding studies using in vitro synthesized myb proteins
  • DNase i footprinting using PCR-generated end-labeled DNA probes
  • Gene Isolation by Screening ?gt11 Expression Libraries with DNA Binding-Site Probes
  • Transcriptional activation analysis by the Chloramphenicol Acetyl Transferase (CAT) enzyme assay.

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