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03235nam a22002895i 4500 |
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978-1-59259-536-5 |
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20191027041507.0 |
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cr nn 008mamaa |
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100301s1995 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592595365
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| 024 |
7 |
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|a 10.1385/089603321X
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 100 |
1 |
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|a Jones, Heddwyn.
|e author.
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| 245 |
1 |
0 |
|a Plant Gene Transfer and Expression Protocols
|c by Heddwyn Jones.
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| 250 |
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|a 1st ed. 1995.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1995.
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| 300 |
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|a XV, 466 p. 118 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 49
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| 505 |
0 |
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|a Agrobacterium-Mediated Transformation -- Tools for Expressing Foreign Genes in Plants -- of Cloning Plasmids into Agrobacterium tumefaciens -- Leaf Disk Transformation Using Agrobacterium tumefaciens-Expression of Heterologous Genes in Tobacco -- Agobacterium rhizogenes as a Vector for Transforming Higher Plants -- Agrobacterium-Mediated Transformation of Arabidopsis thaliana -- Agrobacterium -Mediated Transfer of Geminiviruses to Plant Tissues -- Direct Gene Transfer -- Stable Transformation of Barley via Direct DNA Uptake -- Gene Transfer into Plant Protoplasts by Electroporation -- Transformation of Cereals by Microprojectile Bombardment of Immature Inflorescence and Scutellum Tissues -- Use of Reporter Genes -- The ?-Glucuronidase (gus) Reporter Gene System -- Chloramphenicol Acetyl Transferase Assay -- NPTII Assays for Measuring Gene Expression and Enzyme Activity in Transgenic Plants -- Study of Gene Organization by Southern Blotting and Inverse PCR -- Gene Characterization by Southern Analysis -- Isolation and Characterization of Plant Genomic DNA Sequences via (Inverse) PCR Amplification -- RNA Techniques for Studying Gene Expression -- Isolation of Whole Cell (Total) RNA -- Poly(A)+RNA Isolation -- In Vitro Translation -- Northern Analysis and Nucleic Acid Probes -- Nuclear “Run-On” Transcription Assays -- RNase A/T1 Protection Assay -- Primer Extension Assay -- Applications of RT-PCR -- In Vitro Transcription of Class II Promoters in Higher Plants -- Analysis of Plant Gene Expression by Reverse Transcription-PCR -- In Situ Hybridization to Plant Tissue Sections -- Xenopus Oocytes as a Heterologous Expression System -- Heterologous Expression in Yeast -- Techniques for Studying Chloroplast Gene Expression -- The Isolation of Intact Chloroplasts -- In Vitro Protein Import by Isolated Chloroplasts -- Targeting of Foreign Proteins to the Chloroplast -- Techniques for Studying Mitochondrial Gene Expression -- Isolation of Mitochondria -- Mitochondrial Nucleic Acid Purification and Analysis -- In organello Protein Synthesis -- Immunological Detection of Proteins -- Separation of Plant Proteins by Electrophoresis -- Western Blotting Analysis -- ELISA Detection of Foreign Proteins -- Immunocytochemical Localization of Proteins.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1385/089603321X
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