Plant Gene Transfer and Expression Protocols
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| Corporate Author: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1995.
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| Edition: | 1st ed. 1995. |
| Series: | Methods in Molecular Biology,
49 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/089603321X |
Table of Contents:
- Agrobacterium-Mediated Transformation
- Tools for Expressing Foreign Genes in Plants
- of Cloning Plasmids into Agrobacterium tumefaciens
- Leaf Disk Transformation Using Agrobacterium tumefaciens-Expression of Heterologous Genes in Tobacco
- Agobacterium rhizogenes as a Vector for Transforming Higher Plants
- Agrobacterium-Mediated Transformation of Arabidopsis thaliana
- Agrobacterium -Mediated Transfer of Geminiviruses to Plant Tissues
- Direct Gene Transfer
- Stable Transformation of Barley via Direct DNA Uptake
- Gene Transfer into Plant Protoplasts by Electroporation
- Transformation of Cereals by Microprojectile Bombardment of Immature Inflorescence and Scutellum Tissues
- Use of Reporter Genes
- The ?-Glucuronidase (gus) Reporter Gene System
- Chloramphenicol Acetyl Transferase Assay
- NPTII Assays for Measuring Gene Expression and Enzyme Activity in Transgenic Plants
- Study of Gene Organization by Southern Blotting and Inverse PCR
- Gene Characterization by Southern Analysis
- Isolation and Characterization of Plant Genomic DNA Sequences via (Inverse) PCR Amplification
- RNA Techniques for Studying Gene Expression
- Isolation of Whole Cell (Total) RNA
- Poly(A)+RNA Isolation
- In Vitro Translation
- Northern Analysis and Nucleic Acid Probes
- Nuclear "Run-On" Transcription Assays
- RNase A/T1 Protection Assay
- Primer Extension Assay
- Applications of RT-PCR
- In Vitro Transcription of Class II Promoters in Higher Plants
- Analysis of Plant Gene Expression by Reverse Transcription-PCR
- In Situ Hybridization to Plant Tissue Sections
- Xenopus Oocytes as a Heterologous Expression System
- Heterologous Expression in Yeast
- Techniques for Studying Chloroplast Gene Expression
- The Isolation of Intact Chloroplasts
- In Vitro Protein Import by Isolated Chloroplasts
- Targeting of Foreign Proteins to the Chloroplast
- Techniques for Studying Mitochondrial Gene Expression
- Isolation of Mitochondria
- Mitochondrial Nucleic Acid Purification and Analysis
- In organello Protein Synthesis
- Immunological Detection of Proteins
- Separation of Plant Proteins by Electrophoresis
- Western Blotting Analysis
- ELISA Detection of Foreign Proteins
- Immunocytochemical Localization of Proteins.