Protein Purification Protocols
| Corporate Author: | |
|---|---|
| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1996.
|
| Edition: | 1st ed. 1996. |
| Series: | Methods in Molecular Biology,
59 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/0896033368 |
Table of Contents:
- General Strategies
- Preparation of Extracts from Animal Tissues
- Protein Extraction from Plant Tissues
- Extraction of Recombinant Protein from Bacteria
- Protein Extraction from Fungi
- Subcellular Fractionation of Animal Tissues
- Subcellular Fractionation of Plant Tissues
- The Extraction of Enzymes from Plant Tissues Rich in Phenolic Compounds
- Avoidance of Proteolysis in Extracts
- Concentration of Extracts
- Making and Changing Buffers
- Purification and Concentration by Ultrafiltration
- Bulk Purification by Fractional Precipitation
- Ion-Exchange Chromatography
- Hydrophobic Interaction Chromatography
- Affinity Chromatography
- Dye-Ligand Affinity Chromatography
- Lectin Affinity Chromatography
- Immunoaffinity Chromatography
- Immobilized Metal Ion Affinity Chromatography
- Chromatography on Hydroxyapatite
- Affinity Precipitation Methods
- Isoelectric Focusing
- Chromatofocusing
- Size-Exclusion Chromatography
- Fast Protein Liquid Chromatography (FPLC) Methods
- Reversed-Phase Chromatography of Proteins
- Extraction of Membrane Proteins
- Removal of Detergent from Protein Fractions
- Purification of Membrane Proteins
- Lyophilization of Proteins
- Storage of Pure Proteins
- Electroelution of Proteins from Polyacrylamide Gels
- Electroblotting of Proteins from Polyacrylamide Gels
- High-Performance Electrophoresis Chromatography
- Practical Column Chromatography.