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03406nam a22002895i 4500 |
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978-1-59259-548-8 |
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20191026201816.0 |
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cr nn 008mamaa |
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100301s1997 xxu| s |||| 0|eng d |
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|a 9781592595488
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| 024 |
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|a 10.1385/0896034801
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Recombinant Gene Expression Protocols
|c edited by Rocky S. Tuan.
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| 250 |
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|a 1st ed. 1997.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1997.
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| 300 |
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|a XX, 521 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 62
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|a Recombinant Gene Expression Protocols -- Expression in E. Coli -- Designing Expression Plasmid Vectors in E. coli -- Design of Bacterial Hosts for lac-Based Expression Vectors -- Bacteriophage ?-Based Expression Vectors -- Strategies for Gene Fusions -- Application of the E. coli trp Promoter -- Expression of Proteins in E. coli Utilizing a Dual Promoter-Based Vector: pLACT7 -- Expression and Secretion of Proteins in E. coli -- Synthetic Two-Cistron Expression System -- Expression of cDNA-Encoded Proteins by Cell-Free Transcription and Translation -- Expression in Yeast -- Yeast Plasmids -- Inducible Expression Cassettes in Yeast: GAL4 -- Inducible Expression Cassettes in Yeast: ADH2 -- Constitutive Expression Vectors: PGK -- Viral Expression Systems -- Design and Construction of Recombinant Vaccinia Viruses -- A Simplified Method of Screening for Isolation of Recombinant Vaccinia Virus -- Design of Retroviral Expression Vectors -- Use of Defective Herpes-Derived Plasmid Vectors -- Expression in Nonmammalian Higher Eukaryotes -- Use of Baculovirus Expression Vectors -- Expression of Exogenous Genes in Xenopus Oocytes, Eggs, and Embryos -- Analysis of Heterologous Gene Expression in Xenopus Blastomeres -- Expression in Mammalian Cells -- Overview of Vector Design for Mammalian Gene Expression -- Experimental Strategies in Efficient Transfection of Mammalian Cells -- Experimental Strategies in Efficient Transfection of Mammalian Cells -- Highly Effective Delivery of Foreign DNA to Adherent Cells via Polybrene/DMSO-Assisted Gene Transfer -- Selection of Transfected Cells -- Selection of Transfected Cells and Coamplification of Transfected Genes -- Optimization of Growth, Viability, and Specific Productivity for Expression of Recombinant Proteins in Mammalian Cells -- Expression in Transgenic Organisms -- Promoters to Express Cloned Genes Uniformly in Drosophila -- Application of Micromechanical Piercing Structures for Genetic Transformation of Nematodes -- Strategies in Generating Transgenic Mammals -- Use of Gonadal Primordial Germ Cells (PGCs) as Tools for Gene Transfer in Chickens -- Strategies for the Production of Transgenic Chickens -- Expression in Plants -- Plant Cell Transfection by Electroporation -- Transformation and Foreign Gene Expression in Plants Mediated by Microprojectile Bombardment -- Transformation of the Cereals Using Agrobacterium -- Transformation of Microalgae Using Silicon Carbide Whiskers.
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| 650 |
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|a Cell biology.
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| 650 |
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|a Cell Biology.
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| 700 |
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|a Tuan, Rocky S.
|e editor.
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| 710 |
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|a SpringerLink (Online service)
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/0896034801
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