Recombinant Gene Expression Protocols
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1997.
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| Edición: | 1st ed. 1997. |
| Colección: | Methods in Molecular Biology,
62 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/0896034801 |
Tabla de Contenidos:
- Recombinant Gene Expression Protocols
- Expression in E. Coli
- Designing Expression Plasmid Vectors in E. coli
- Design of Bacterial Hosts for lac-Based Expression Vectors
- Bacteriophage ?-Based Expression Vectors
- Strategies for Gene Fusions
- Application of the E. coli trp Promoter
- Expression of Proteins in E. coli Utilizing a Dual Promoter-Based Vector: pLACT7
- Expression and Secretion of Proteins in E. coli
- Synthetic Two-Cistron Expression System
- Expression of cDNA-Encoded Proteins by Cell-Free Transcription and Translation
- Expression in Yeast
- Yeast Plasmids
- Inducible Expression Cassettes in Yeast: GAL4
- Inducible Expression Cassettes in Yeast: ADH2
- Constitutive Expression Vectors: PGK
- Viral Expression Systems
- Design and Construction of Recombinant Vaccinia Viruses
- A Simplified Method of Screening for Isolation of Recombinant Vaccinia Virus
- Design of Retroviral Expression Vectors
- Use of Defective Herpes-Derived Plasmid Vectors
- Expression in Nonmammalian Higher Eukaryotes
- Use of Baculovirus Expression Vectors
- Expression of Exogenous Genes in Xenopus Oocytes, Eggs, and Embryos
- Analysis of Heterologous Gene Expression in Xenopus Blastomeres
- Expression in Mammalian Cells
- Overview of Vector Design for Mammalian Gene Expression
- Experimental Strategies in Efficient Transfection of Mammalian Cells
- Experimental Strategies in Efficient Transfection of Mammalian Cells
- Highly Effective Delivery of Foreign DNA to Adherent Cells via Polybrene/DMSO-Assisted Gene Transfer
- Selection of Transfected Cells
- Selection of Transfected Cells and Coamplification of Transfected Genes
- Optimization of Growth, Viability, and Specific Productivity for Expression of Recombinant Proteins in Mammalian Cells
- Expression in Transgenic Organisms
- Promoters to Express Cloned Genes Uniformly in Drosophila
- Application of Micromechanical Piercing Structures for Genetic Transformation of Nematodes
- Strategies in Generating Transgenic Mammals
- Use of Gonadal Primordial Germ Cells (PGCs) as Tools for Gene Transfer in Chickens
- Strategies for the Production of Transgenic Chickens
- Expression in Plants
- Plant Cell Transfection by Electroporation
- Transformation and Foreign Gene Expression in Plants Mediated by Microprojectile Bombardment
- Transformation of the Cereals Using Agrobacterium
- Transformation of Microalgae Using Silicon Carbide Whiskers.