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03548nam a22002895i 4500 |
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978-1-59259-549-5 |
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20191026201815.0 |
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cr nn 008mamaa |
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100301s1997 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592595495
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| 024 |
7 |
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|a 10.1385/089603481X
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Recombinant Protein Protocols
|b Detection and Isolation /
|c edited by Rocky S. Tuan.
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| 250 |
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|a 1st ed. 1997.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1997.
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| 300 |
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|a XX, 448 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 63
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| 505 |
0 |
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|a Overview of Experimental Strategies on the Detection and Isolation of Recombinant Proteins and Their Applications -- Detection and Isolation of Expressed Proteins -- Reporter Systems -- Detection of Recombinant Protein Based on Reporter Enzyme Activity: Chloramphenicol Acetyltransferase -- Human Placental Alkaline Phosphatase as a Marker for Gene Expression -- Use of Secreted Alkaline Phosphatase as a Reporter of Gene Expression in Mammalian Cells -- Detection of ?-Galactosidase and ?-Glucuronidase Using Chemiluminescent Reporter Gene Assays -- Chemiluminescent Immunoassay for the Detection of Chloramphenicol Acetyltransferase and Human Growth Hormone Reporter Proteins -- Detection and Selection of Cultured Cells Secreting Recombinant Product by Soft Agar Cloning and Antibody Overlay -- Detection and Isolation of Recombinant Protein Based on Binding Affinity Reporter -- Detection and Isolation of Recombinant Proteins Based on Binding Affinity of Reporter: Protein A -- Expression and Purification of Recombinant Streptavidin-Containing Chimeric Proteins -- Bacterial Expression, Purification, and Potential Use of His-Tagged GAL4 Fusion Proteins -- Detection of Expressed Recombinant Protein Based on Multidrug Resistance: P-Glycoprotein -- Detection and Isolation of Recombinant Proteins from Mammalian Cells by Immunoaffinity Chromatography: p53 -- Yeast GAL4 Two-Hybrid System -- Alternative Yeast Two-Hybrid Systems -- Detection of Heterologous Gs-Coupled Receptor Activity in LLC-PK1 Cells Based on Expression of Urokinase-Type Plasminogen Activator -- Detection of Recombinant Gene Expression In Situ -- Histochemical and Fluorochrome-Based Detection of ?-Galactosidase -- Combined, Sequential In Situ Hybridization and Immunohistochemistry on the Same Tissue Section -- Whole Mount In Situ Hybridization to Embryos and Embryonic Tissues -- Whole-Mount In Situ Hybridization for Developing Chick Embryos Using Digoxygenin-Labeled RNA Probes -- Methods for Double Detection of Gene Expression -- In Situ PCR -- Expression and Detection of Green Fluorescent Protein (GFP) -- Applications of Recombinant Gene Expression -- Transgenic Animals as Bioreactors for Expression of Recombinant Proteins -- Artificial Cells for Bioencapsulation of Cells and Genetically Engineered E. coli -- Hyperexpression of a Synthetic Protein-Based Polymer Gene -- Transplantation of Cells in an immunoisolation Device for Gene Therapy -- Delivery of Recombinant HIV-1-Directed Antisense and Ribozyme Genes -- Inducible System Designed for Future Gene Therapy -- Human Gene Therapy.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Tuan, Rocky S.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/089603481X
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