Recombinant Protein Protocols Detection and Isolation /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1997.
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| Edición: | 1st ed. 1997. |
| Colección: | Methods in Molecular Biology,
63 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/089603481X |
Tabla de Contenidos:
- Overview of Experimental Strategies on the Detection and Isolation of Recombinant Proteins and Their Applications
- Detection and Isolation of Expressed Proteins
- Reporter Systems
- Detection of Recombinant Protein Based on Reporter Enzyme Activity: Chloramphenicol Acetyltransferase
- Human Placental Alkaline Phosphatase as a Marker for Gene Expression
- Use of Secreted Alkaline Phosphatase as a Reporter of Gene Expression in Mammalian Cells
- Detection of ?-Galactosidase and ?-Glucuronidase Using Chemiluminescent Reporter Gene Assays
- Chemiluminescent Immunoassay for the Detection of Chloramphenicol Acetyltransferase and Human Growth Hormone Reporter Proteins
- Detection and Selection of Cultured Cells Secreting Recombinant Product by Soft Agar Cloning and Antibody Overlay
- Detection and Isolation of Recombinant Protein Based on Binding Affinity Reporter
- Detection and Isolation of Recombinant Proteins Based on Binding Affinity of Reporter: Protein A
- Expression and Purification of Recombinant Streptavidin-Containing Chimeric Proteins
- Bacterial Expression, Purification, and Potential Use of His-Tagged GAL4 Fusion Proteins
- Detection of Expressed Recombinant Protein Based on Multidrug Resistance: P-Glycoprotein
- Detection and Isolation of Recombinant Proteins from Mammalian Cells by Immunoaffinity Chromatography: p53
- Yeast GAL4 Two-Hybrid System
- Alternative Yeast Two-Hybrid Systems
- Detection of Heterologous Gs-Coupled Receptor Activity in LLC-PK1 Cells Based on Expression of Urokinase-Type Plasminogen Activator
- Detection of Recombinant Gene Expression In Situ
- Histochemical and Fluorochrome-Based Detection of ?-Galactosidase
- Combined, Sequential In Situ Hybridization and Immunohistochemistry on the Same Tissue Section
- Whole Mount In Situ Hybridization to Embryos and Embryonic Tissues
- Whole-Mount In Situ Hybridization for Developing Chick Embryos Using Digoxygenin-Labeled RNA Probes
- Methods for Double Detection of Gene Expression
- In Situ PCR
- Expression and Detection of Green Fluorescent Protein (GFP)
- Applications of Recombinant Gene Expression
- Transgenic Animals as Bioreactors for Expression of Recombinant Proteins
- Artificial Cells for Bioencapsulation of Cells and Genetically Engineered E. coli
- Hyperexpression of a Synthetic Protein-Based Polymer Gene
- Transplantation of Cells in an immunoisolation Device for Gene Therapy
- Delivery of Recombinant HIV-1-Directed Antisense and Ribozyme Genes
- Inducible System Designed for Future Gene Therapy
- Human Gene Therapy.