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02867nam a22002895i 4500 |
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978-1-59259-550-1 |
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20191027042403.0 |
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cr nn 008mamaa |
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100301s1997 xxu| s |||| 0|eng d |
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|a 9781592595501
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| 024 |
7 |
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|a 10.1385/0896033538
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Protein Sequencing Protocols
|c edited by Bryan John Smith.
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| 250 |
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|a 1st ed. 1997.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1997.
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| 300 |
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|a XIV, 375 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 64
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|a Strategies for Handling Polypeptides on a Microscale -- SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing -- Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization -- Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization -- Enzymatic Cleavage of Polypeptides -- Chemical Cleavage of Polypeptides -- Preparation of Glycopeptides -- Selective Isolation of the Carboxy Terminal Peptide from a Protein -- Selective Isolation of the Amino-Terminal Peptide from ?-Amino Blocked Protein -- Analytical and Micropreparative Capillary Electrophoresis of Peptides -- Reverse-Phase HPLC Purification of Peptides from Natural Sources for Structural Analysis -- High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues -- Hydrolysis of Samples for Amino Acid Analysis -- Amino Acid Analysis -- Postcolumn Amino Acid Analysis -- Fast-Atom Bombardment and Electrospray Mass Spectrometry of Peptides, Proteins, and Glycoproteins -- Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry -- N-Terminal Protein Ladder Sequencing -- The Dansyl-Edman Method for Manual Peptide Sequencing -- The Dansyl Method for Identifying N-Terminal Amino Acids -- Identification of PTH-Amino Acids by High-Performance Liquid Chromatography -- Protein Sequencer Maintenance and Troubleshooting -- Identifying the Carboxy Terminal Amino Acid of a Protein -- Enzymatic and Chemical Methods for Manual C-Terminal Peptide Sequencing -- Automated Methods for C-Terminal Protein Sequencing -- Chemical Modification of Proteins for Sequence Analysis -- On-Membrane Deblocking of Proteins -- Analysis of Sites of Protein Phosphorylation -- Identification of Sites of Glycosylation -- Quantification and Location of Disulfide Bonds in Proteins.
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| 650 |
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|a Analytical chemistry.
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| 650 |
1 |
4 |
|a Analytical Chemistry.
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| 700 |
1 |
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|a Smith, Bryan John.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/0896033538
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