Protein Sequencing Protocols
| Corporate Author: | |
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1997.
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| Edition: | 1st ed. 1997. |
| Series: | Methods in Molecular Biology,
64 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/0896033538 |
Table of Contents:
- Strategies for Handling Polypeptides on a Microscale
- SDS Polyacrylamide Gel Electrophoresis for N-Terminal Protein Sequencing
- Two-Dimensional Polyacrylamide Gel Electrophoresis for the Separation of Proteins for Chemical Characterization
- Electroblotting of Proteins from Polyacrylamide Gels for Chemical Characterization
- Enzymatic Cleavage of Polypeptides
- Chemical Cleavage of Polypeptides
- Preparation of Glycopeptides
- Selective Isolation of the Carboxy Terminal Peptide from a Protein
- Selective Isolation of the Amino-Terminal Peptide from ?-Amino Blocked Protein
- Analytical and Micropreparative Capillary Electrophoresis of Peptides
- Reverse-Phase HPLC Purification of Peptides from Natural Sources for Structural Analysis
- High-Performance Liquid Chromatography On-Line Derivative Spectroscopy for the Characterization of Peptides with Aromatic Amino Acid Residues
- Hydrolysis of Samples for Amino Acid Analysis
- Amino Acid Analysis
- Postcolumn Amino Acid Analysis
- Fast-Atom Bombardment and Electrospray Mass Spectrometry of Peptides, Proteins, and Glycoproteins
- Peptide Mass Fingerprinting Using MALDI-TOF Mass Spectrometry
- N-Terminal Protein Ladder Sequencing
- The Dansyl-Edman Method for Manual Peptide Sequencing
- The Dansyl Method for Identifying N-Terminal Amino Acids
- Identification of PTH-Amino Acids by High-Performance Liquid Chromatography
- Protein Sequencer Maintenance and Troubleshooting
- Identifying the Carboxy Terminal Amino Acid of a Protein
- Enzymatic and Chemical Methods for Manual C-Terminal Peptide Sequencing
- Automated Methods for C-Terminal Protein Sequencing
- Chemical Modification of Proteins for Sequence Analysis
- On-Membrane Deblocking of Proteins
- Analysis of Sites of Protein Phosphorylation
- Identification of Sites of Glycosylation
- Quantification and Location of Disulfide Bonds in Proteins.