cDNA Library Protocols
| Corporate Author: | |
|---|---|
| Other Authors: | , |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1997.
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| Edition: | 1st ed. 1997. |
| Series: | Methods in Molecular Biology,
69 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/089603383X |
Table of Contents:
- cDNA Library Construction from Small Amounts of RNA Using Paramagnetic Beads and PCR
- Increasing the Average Abundance of Low-Abundance cDNAs by Ordered Subdivision of cDNA Populations
- Isolation of Messenger RNA from Plant Tissues
- cDNA Library Construction for the Lambda ZAP®-Based Vectors
- Clone Excision Methods for the Lambda ZAP®-Based Vectors
- Using Rapid Amplification of cDNA Ends (RACE) to Obtain Full-Length cDNAs
- Inverse PCR Approach to Cloning cDNA Ends
- Cloning Gene Family Members Using the Polymerase Chain Reaction with Degenerate Oligonucleotide Primers
- Subtractive Hybridization for the Isolation of Differentially Expressed Genes Using Magnetic Beads
- Preparation of Competent Cells for High-Efficiency Plasmid Transformation of Escherichia coli
- The Tetramethylammonium Chloride (TMAC) Method for Screening cDNA Libraries with Highly Degenerate Oligonucleotide Probes Obtained by Reverse Translation of Amino Acid Sequences
- Screening cDNA Libraries by Hybridization with Double-Stranded DNA Probes and Oligonucleotides
- Immunological Screening of ?-Phage cDNA Expression Libraries
- Cloning Sequence-Specific DNA-Binding Factors from cDNA Expression Libraries Using Oligonucleotide Binding Site Probes
- Protein Interaction Cloning by Far-Western Screening of ?-Phage cDNA Expression Libraries
- Yeast Two-Hybrid Library Screening
- Signal Sequence Trap
- Isolation of Genetic Suppressor Elements (GSEs) from Random Fragment cDNA Libraries in Retroviral Vectors
- Expression and Preparation of Fusion Proteins from Recombinant ?gt1.1 Phages
- Computer Analysis of Cloned Sequences
- The TIGR Human cDNA Database
- Searching the dbEST Database
- Reference cDNA Library Facilities Available from European Sources.