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03434nam a22003015i 4500 |
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978-1-59259-559-4 |
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20190618131531.0 |
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cr nn 008mamaa |
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100301s1997 xxu| s |||| 0|eng d |
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|a 9781592595594
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| 024 |
7 |
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|a 10.1385/0896033996
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Neuropeptide Protocols
|c edited by G. Brent Irvine, Carvell H. Williams.
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| 250 |
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|a 1st ed. 1997.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1997.
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| 300 |
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|a XIII, 386 p. 22 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 73
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|a Preparation of Neuropeptide-Containing Fractions from Biological Materials -- Purification of Extracted Peptides for Structural Analysis -- Amino Acid Sequencing of Neuropeptides -- Neuropeptide Gene Identification Using the Polymerase Chain Reaction -- Solid-Phase Synthesis of Neuropeptides by Fmoc Strategies -- Incorporation of Stable Pseudopeptide Bonds -- Synthesis of Conformationally Restricted Peptides -- Purification of Synthetic Peptides by High Performance Liquid Chromatography -- Molecular Weight Estimation for Neuropeptides Using Size-Exclusion High Performance Liquid Chromatography -- Molecular Weight Determinations Using Polyacrylamide Gel Electrophoresis with Tris-Tricine Buffers -- Determination of Neuropeptides by Capillary Electrophoresis -- Characterization of Neuropeptide Processing by Fast Atom Bombardment Mass Spectrometry -- Analysis of Neuropeptides by Size-Exclusion HPLC Linked to Electrospray Ionization Mass Spectrometry -- Identification of Peptides by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS) and Direct Analysis of the Laterobuccal Nerve from the Pond Snail Lymnaea stagnalis -- Use of Circular Dichroism to Determine Secondary Structure of Neuropeptides -- 1H Nuclear Magnetic Resonance (NMR) in the Elucidation of Peptide Structure -- The Study of Membrane- or Receptor-Bound Neuropeptides by NMR -- Molecular Modeling of Neuropeptides -- Tritium Labeling of Neuropeptides -- The Use of IODO-GEN for Preparing 125I-Labeled Peptides and Their Purification by Reversed-Phase High Performance Liquid Chromatography -- Production of Antisera Using Peptide Conjugates -- Radioimmunoassay -- Enzyme-Linked Immunosorbent Assay of Peptides -- Sample Preparation for Peptide Immunocytochemistry -- lmmunocytochemical Methods for Regulatory Peptides -- Ultrastructural Localization of Peptides Using Immunogold Labeling -- Preparation of a Membrane Fraction for Receptor Studies and Solubilization of Receptor Proteins with Retention of Biological Activity -- Radioligand Binding Using 125Labeled Peptides -- Analysis of Data from “Cold Saturation” Radioligand Binding Experiments -- Organ/Tissue Preparations for the Assessment of Agonist/Antagonist Activity -- Measurement of Efflux Rates from Brain to Blood -- Assay of Neuropeptidases Using Fluorogenic Substrates -- Characterization of Neuropeptidases Using Inhibitors.
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| 650 |
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|a Neurosciences.
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| 650 |
1 |
4 |
|a Neurosciences.
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| 700 |
1 |
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|a Irvine, G. Brent.
|e editor.
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| 700 |
1 |
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|a Williams, Carvell H.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/0896033996
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