Neuropeptide Protocols
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1997.
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| Edición: | 1st ed. 1997. |
| Colección: | Methods in Molecular Biology,
73 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/0896033996 |
Tabla de Contenidos:
- Preparation of Neuropeptide-Containing Fractions from Biological Materials
- Purification of Extracted Peptides for Structural Analysis
- Amino Acid Sequencing of Neuropeptides
- Neuropeptide Gene Identification Using the Polymerase Chain Reaction
- Solid-Phase Synthesis of Neuropeptides by Fmoc Strategies
- Incorporation of Stable Pseudopeptide Bonds
- Synthesis of Conformationally Restricted Peptides
- Purification of Synthetic Peptides by High Performance Liquid Chromatography
- Molecular Weight Estimation for Neuropeptides Using Size-Exclusion High Performance Liquid Chromatography
- Molecular Weight Determinations Using Polyacrylamide Gel Electrophoresis with Tris-Tricine Buffers
- Determination of Neuropeptides by Capillary Electrophoresis
- Characterization of Neuropeptide Processing by Fast Atom Bombardment Mass Spectrometry
- Analysis of Neuropeptides by Size-Exclusion HPLC Linked to Electrospray Ionization Mass Spectrometry
- Identification of Peptides by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS) and Direct Analysis of the Laterobuccal Nerve from the Pond Snail Lymnaea stagnalis
- Use of Circular Dichroism to Determine Secondary Structure of Neuropeptides
- 1H Nuclear Magnetic Resonance (NMR) in the Elucidation of Peptide Structure
- The Study of Membrane- or Receptor-Bound Neuropeptides by NMR
- Molecular Modeling of Neuropeptides
- Tritium Labeling of Neuropeptides
- The Use of IODO-GEN for Preparing 125I-Labeled Peptides and Their Purification by Reversed-Phase High Performance Liquid Chromatography
- Production of Antisera Using Peptide Conjugates
- Radioimmunoassay
- Enzyme-Linked Immunosorbent Assay of Peptides
- Sample Preparation for Peptide Immunocytochemistry
- lmmunocytochemical Methods for Regulatory Peptides
- Ultrastructural Localization of Peptides Using Immunogold Labeling
- Preparation of a Membrane Fraction for Receptor Studies and Solubilization of Receptor Proteins with Retention of Biological Activity
- Radioligand Binding Using 125Labeled Peptides
- Analysis of Data from “Cold Saturation” Radioligand Binding Experiments
- Organ/Tissue Preparations for the Assessment of Agonist/Antagonist Activity
- Measurement of Efflux Rates from Brain to Blood
- Assay of Neuropeptidases Using Fluorogenic Substrates
- Characterization of Neuropeptidases Using Inhibitors.