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03051nam a22002895i 4500 |
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978-1-59259-563-1 |
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20190618131531.0 |
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cr nn 008mamaa |
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100301s1998 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592595631
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| 024 |
7 |
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|a 10.1385/089603397X
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Protein Synthesis
|b Methods and Protocols /
|c edited by Robin Martin.
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| 250 |
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|a 1st ed. 1998.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1998.
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| 300 |
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|a XIV, 442 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 77
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|a Analyses of Ribosome Distribution During In Vitro Translation -- Allele-Specific Priming in the Mapping of rRNA -- Permeabilized Mammalian Cells as a System for Protein Synthesis -- The Measurement of Processivity Errors in Protein Synthesis -- A Biosynthetic Approach for the Incorporation of Unnatural Amino Acids into Proteins -- The Analysis of Translational Activity Using a Reporter Gene Constructed from Repeats of an Antibody-Binding Domain from Protein A -- In Vitro Engineering Using Acyl-Derivatized tRNAs -- In Vitro Engineering Using Synthetic tRNAs with Altered Anticodons Including Four-Nucleotide Anticodons -- Analysis of rRNA Function Using Specialized Ribosomes -- Determination of the Peptide Elongation Rate In Vivo -- The Measurement of Missense Errors During In Vivo Protein Synthesis -- Heelprinting Analysis of In Vivo Ribosome Pause Sites -- Analysis of elF-2? Kinases in Yeast -- Continuous-Flow Cell-Free Translation, Transcription-Translation, and Replication-Translation Systems -- Analysis of Translational Activity of Extracts Derived from Oocytes and Eggs of Xenopus laevis -- A Fractionated Reticulocyte Lysate System for Studies on Protein Synthesis Initiation Factors -- Measurement of Ribosomal Accuracy and Proofreading in E. coli Burst Systems -- Measurement of Rate of Protein Synthesis In Vitro -- In Vivo Mutational Analysis of Ribosomal RNA in Saccharomyces cerevisiae -- Genetic Selection of rRNA Mutations -- Toeprinting Assays -- Mapping of Pseudouridine Residues in RNA to Nucleotide Resolution -- Chemical and Enzymatic Probing of Antibiotic-Ribosome Complexes -- Protein Engineering with Nonstandard Amino Acids -- Internal Ribosome Entry Sites Tests with Circular mRNAs -- Gel Retardation and UV-Crosslinking Assays to Detect Specific RNA-Protein Interactions in the 5? or 3? UTRs of Translationally Regulated mRNAs -- Translational Control by Repressor Proteins Binding to the 5?UTR of mRNAs -- Identification and Analysis of Frameshift Sites -- Synthesis and Site-Specific Binding of Thioated tRNAs to Probe Ribosome-tRNA Interactions.
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| 650 |
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|a Biochemistry.
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| 650 |
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|a Biochemistry, general.
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| 700 |
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|a Martin, Robin.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/089603397X
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