Protein Synthesis Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1998.
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| Edición: | 1st ed. 1998. |
| Colección: | Methods in Molecular Biology,
77 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/089603397X |
Tabla de Contenidos:
- Analyses of Ribosome Distribution During In Vitro Translation
- Allele-Specific Priming in the Mapping of rRNA
- Permeabilized Mammalian Cells as a System for Protein Synthesis
- The Measurement of Processivity Errors in Protein Synthesis
- A Biosynthetic Approach for the Incorporation of Unnatural Amino Acids into Proteins
- The Analysis of Translational Activity Using a Reporter Gene Constructed from Repeats of an Antibody-Binding Domain from Protein A
- In Vitro Engineering Using Acyl-Derivatized tRNAs
- In Vitro Engineering Using Synthetic tRNAs with Altered Anticodons Including Four-Nucleotide Anticodons
- Analysis of rRNA Function Using Specialized Ribosomes
- Determination of the Peptide Elongation Rate In Vivo
- The Measurement of Missense Errors During In Vivo Protein Synthesis
- Heelprinting Analysis of In Vivo Ribosome Pause Sites
- Analysis of elF-2? Kinases in Yeast
- Continuous-Flow Cell-Free Translation, Transcription-Translation, and Replication-Translation Systems
- Analysis of Translational Activity of Extracts Derived from Oocytes and Eggs of Xenopus laevis
- A Fractionated Reticulocyte Lysate System for Studies on Protein Synthesis Initiation Factors
- Measurement of Ribosomal Accuracy and Proofreading in E. coli Burst Systems
- Measurement of Rate of Protein Synthesis In Vitro
- In Vivo Mutational Analysis of Ribosomal RNA in Saccharomyces cerevisiae
- Genetic Selection of rRNA Mutations
- Toeprinting Assays
- Mapping of Pseudouridine Residues in RNA to Nucleotide Resolution
- Chemical and Enzymatic Probing of Antibiotic-Ribosome Complexes
- Protein Engineering with Nonstandard Amino Acids
- Internal Ribosome Entry Sites Tests with Circular mRNAs
- Gel Retardation and UV-Crosslinking Assays to Detect Specific RNA-Protein Interactions in the 5? or 3? UTRs of Translationally Regulated mRNAs
- Translational Control by Repressor Proteins Binding to the 5?UTR of mRNAs
- Identification and Analysis of Frameshift Sites
- Synthesis and Site-Specific Binding of Thioated tRNAs to Probe Ribosome-tRNA Interactions.