Plant Virology Protocols From Virus Isolation to Transgenic Resistance /
| Corporate Author: | |
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| Other Authors: | , |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1998.
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| Edition: | 1st ed. 1998. |
| Series: | Methods in Molecular Biology,
81 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/0896033856 |
Table of Contents:
- to Plant Virology
- to Classical Crossprotection
- History of Coat Protein-Mediated Protection
- Virus Isolation, RNA Extraction, and Location of Coat Protein Genes
- Geminivirus Isolation and DNA Extraction
- Caulimovirus Isolation and DNA Extraction
- Reovirus Isolation and RNA Extraction
- Procedures for Plant Rhabdovirus Purification, Polyribosome Isolation, and Replicase Extraction
- Hordeivirus Isolation and RNA Extraction
- Furovirus Isolation and RNA Extraction
- Tobravirus Isolation and RNA Extraction
- Tobamovirus Isolation and RNA Extraction
- Potexvirus Isolation and RNA Extraction
- Carlavirus Isolation and RNA Extraction
- Potyvirus Isolation and RNA Extraction
- Trichovirus Isolation and RNA Extraction
- Ilarvirus Isolation and RNA Extraction
- Bromovirus Isolation and RNA Extraction
- Cucumovirus Isolation and RNA Extraction
- Nepovirus Isolation and RNA Extraction
- Comovirus Isolation and RNA Extraction
- Carmovirus Isolation and RNA Extraction
- Tymovirus Isolation and Genomic RNA Extraction
- Tombusvirus Isolation and RNA Extraction
- Luteovirus Isolation and RNA Extraction
- Cloning of Coat Protein Genes
- RNA Analysis
- RNA Fractionation by Density Gradient Centrifugation
- cDNA Library Construction for the Lambda ZAP®-Based Vectors
- PCR Cloning of Coat Protein Genes
- Antibody Production
- Expression Library Screening
- In Vitro Transcription and Translation
- Analysis of Coat Protein Expression Cassettes in Protoplasts
- DNA Sequencing
- Computer Analysis of Amino Acid Sequences
- Plant Transformation and Primary Analysis
- Preparation of Coat Protein-Containing Binary Vectors for Use in Agrobacterium-Mediated Transformation
- Potato Transformation
- Transformation of Tomato
- Tobacco Transformation
- Genetic Transformation of Wheat
- Production of Transgenic Rice (Oryza sativa subspecies japonica cv. Taipei 309)
- Molecular Analysis of Transgenic Rice
- PCR Analysis of Transgenic Tobacco Plants
- Southern Analysis of Transgenic Tobacco Plants
- Detection and Quantification of Transcript RNA in Transgenic Plants Using Digoxigenin-Labeled cDNA Probes
- Western Analysis of Transgenic Plants
- Evaluation of Resistance
- Assaying Levels of Plant Virus by ELISA
- Detection of Plant RNA Viruses by Nonisotopic Dot-Blot Hybridization
- Detection and Quantification of Plant Viruses by PCR
- Assaying Levels of Virus with Local Lesion Hosts
- Field Testing Resistance of Transgenic Plants
- Agronomic Performance of Transgenic Plants
- Mechanisms of Resistance
- Mechanisms of Resistance
- Mechanisms of RNA-Mediated Resistance to Plant Viruses
- Detection of Risks Associated with Coat Protein Transgenics
- Potential Benefits of the Transgenic Control of Plant Viruses in the United Kingdom.