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03287nam a22002895i 4500 |
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978-1-59259-573-0 |
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20191027051709.0 |
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cr nn 008mamaa |
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100301s1998 xxu| s |||| 0|eng d |
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|a 9781592595730
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024 |
7 |
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|a 10.1385/0896034380
|2 doi
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040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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245 |
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|a Retinoid Protocols
|c edited by Christopher Redfern.
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250 |
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|a 1st ed. 1998.
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260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1998.
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300 |
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|a XVI, 434 p.
|b online resource.
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336 |
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|a text
|b txt
|2 rdacontent
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337 |
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|a computer
|b c
|2 rdamedia
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338 |
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|a online resource
|b cr
|2 rdacarrier
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490 |
1 |
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|a Methods in Molecular Biology,
|v 89
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|a Handling and Analysis of Retinoids -- Properties of Retinoids -- Quantitative Analyses of Naturally Occurring Retinoids -- Detection and Measurement of Retinoic Acid Production by Isolated Tissues Using Retinoic Acid-Sensitive Reporter Cell Lines -- Retinoid-Binding Proteins -- Immunohistochemistry for CRBPs and CRABPs -- Whole-Mount In Situ Hybridization of Mouse Embryos Exposed to Teratogenic Levels of Retinoic Acid -- Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) for Cellular Retinoid-Binding Proteins -- Methods for Producing Recombinant Human Cellular Retinaldehyde-Binding Protein -- Expression and Purification of CRABPs from E. coli -- Purification and Fluorescent Titration of Cellular Retinol-Binding Protein -- Fluorometric Titration of the CRABPs -- Expression and Mutagenesis of Retinol-Binding Protein -- Interactions of Retinol-Binding Protein with Transthyretin and Its Receptor -- Detection of Conformational Changes in Cellular Retinoid-Binding Proteins by Limited Proteolysis -- Measurement of Rates of Dissociation of Retinoids from the Interphotoreceptor Retinoid-Binding Protein -- Use of Antisense Oligonucleotides to Study the Role of CRABPs in Retinoic Acid-Induced Gene Expression -- Nuclear Retinoid Receptors -- Preparation of Polyclonal Antibodies to Retinoid Receptors -- Detection of RARs and RXRs in Cells and Tissues Using Specific Ligand-Binding Assays and Ligand-Binding lmmunoprecipitation Techniques -- Nonisotopic In Situ Hybridization for the Detection of Nuclear Retinoid Receptor Transcripts in Tissue Sections -- In Situ Hybridization with 35S-Labeled Probes for Retinoid Receptors -- Isolation of Retinoid Receptors from Manimalian Cells -- Analysis of Retinoid Receptor Phosphorylation -- Photoaffinity Labeling of RARs and Mapping of Labeled Sites by an Endoproteinase Combination Technique -- PCR Cloning of N-Terminal RAR Isoforms and APL-Associated PLZF-RAR? Fusion Proteins -- RT-PCR in Diagnosis and Disease Monitoring of Acute Promyelocytic Leukemia (APL) -- A Two-Hybrid Protein Interaction System to Identify Factors That Interact with Retinoid and Vitamin D Receptors -- Gel-Shift Analysis and Identification of RXREs and RAREs by PCR-Based Selection -- Identification and Cloning of RA-Regulated Genes by mRNA-Differential Display -- Gene Targeting of Retinoid Receptors.
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650 |
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|a Cell biology.
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650 |
1 |
4 |
|a Cell Biology.
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700 |
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|a Redfern, Christopher.
|e editor.
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710 |
2 |
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|a SpringerLink (Online service)
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|t Springer eBooks
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856 |
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|u https://doi.org/10.1385/0896034380
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