Retinoid Protocols

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Redfern, Christopher. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 1998.
Edición:1st ed. 1998.
Colección:Methods in Molecular Biology, 89
Materias:
Acceso en línea:https://doi.org/10.1385/0896034380
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245 1 0 |a Retinoid Protocols  |c edited by Christopher Redfern. 
250 |a 1st ed. 1998. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 1998. 
300 |a XVI, 434 p.  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
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490 1 |a Methods in Molecular Biology,  |v 89 
505 0 |a Handling and Analysis of Retinoids -- Properties of Retinoids -- Quantitative Analyses of Naturally Occurring Retinoids -- Detection and Measurement of Retinoic Acid Production by Isolated Tissues Using Retinoic Acid-Sensitive Reporter Cell Lines -- Retinoid-Binding Proteins -- Immunohistochemistry for CRBPs and CRABPs -- Whole-Mount In Situ Hybridization of Mouse Embryos Exposed to Teratogenic Levels of Retinoic Acid -- Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) for Cellular Retinoid-Binding Proteins -- Methods for Producing Recombinant Human Cellular Retinaldehyde-Binding Protein -- Expression and Purification of CRABPs from E. coli -- Purification and Fluorescent Titration of Cellular Retinol-Binding Protein -- Fluorometric Titration of the CRABPs -- Expression and Mutagenesis of Retinol-Binding Protein -- Interactions of Retinol-Binding Protein with Transthyretin and Its Receptor -- Detection of Conformational Changes in Cellular Retinoid-Binding Proteins by Limited Proteolysis -- Measurement of Rates of Dissociation of Retinoids from the Interphotoreceptor Retinoid-Binding Protein -- Use of Antisense Oligonucleotides to Study the Role of CRABPs in Retinoic Acid-Induced Gene Expression -- Nuclear Retinoid Receptors -- Preparation of Polyclonal Antibodies to Retinoid Receptors -- Detection of RARs and RXRs in Cells and Tissues Using Specific Ligand-Binding Assays and Ligand-Binding lmmunoprecipitation Techniques -- Nonisotopic In Situ Hybridization for the Detection of Nuclear Retinoid Receptor Transcripts in Tissue Sections -- In Situ Hybridization with 35S-Labeled Probes for Retinoid Receptors -- Isolation of Retinoid Receptors from Manimalian Cells -- Analysis of Retinoid Receptor Phosphorylation -- Photoaffinity Labeling of RARs and Mapping of Labeled Sites by an Endoproteinase Combination Technique -- PCR Cloning of N-Terminal RAR Isoforms and APL-Associated PLZF-RAR? Fusion Proteins -- RT-PCR in Diagnosis and Disease Monitoring of Acute Promyelocytic Leukemia (APL) -- A Two-Hybrid Protein Interaction System to Identify Factors That Interact with Retinoid and Vitamin D Receptors -- Gel-Shift Analysis and Identification of RXREs and RAREs by PCR-Based Selection -- Identification and Cloning of RA-Regulated Genes by mRNA-Differential Display -- Gene Targeting of Retinoid Receptors. 
650 0 |a Cell biology. 
650 1 4 |a Cell Biology. 
700 1 |a Redfern, Christopher.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
856 4 0 |u https://doi.org/10.1385/0896034380