Retinoid Protocols
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1998.
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| Edición: | 1st ed. 1998. |
| Colección: | Methods in Molecular Biology,
89 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/0896034380 |
Tabla de Contenidos:
- Handling and Analysis of Retinoids
- Properties of Retinoids
- Quantitative Analyses of Naturally Occurring Retinoids
- Detection and Measurement of Retinoic Acid Production by Isolated Tissues Using Retinoic Acid-Sensitive Reporter Cell Lines
- Retinoid-Binding Proteins
- Immunohistochemistry for CRBPs and CRABPs
- Whole-Mount In Situ Hybridization of Mouse Embryos Exposed to Teratogenic Levels of Retinoic Acid
- Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) for Cellular Retinoid-Binding Proteins
- Methods for Producing Recombinant Human Cellular Retinaldehyde-Binding Protein
- Expression and Purification of CRABPs from E. coli
- Purification and Fluorescent Titration of Cellular Retinol-Binding Protein
- Fluorometric Titration of the CRABPs
- Expression and Mutagenesis of Retinol-Binding Protein
- Interactions of Retinol-Binding Protein with Transthyretin and Its Receptor
- Detection of Conformational Changes in Cellular Retinoid-Binding Proteins by Limited Proteolysis
- Measurement of Rates of Dissociation of Retinoids from the Interphotoreceptor Retinoid-Binding Protein
- Use of Antisense Oligonucleotides to Study the Role of CRABPs in Retinoic Acid-Induced Gene Expression
- Nuclear Retinoid Receptors
- Preparation of Polyclonal Antibodies to Retinoid Receptors
- Detection of RARs and RXRs in Cells and Tissues Using Specific Ligand-Binding Assays and Ligand-Binding lmmunoprecipitation Techniques
- Nonisotopic In Situ Hybridization for the Detection of Nuclear Retinoid Receptor Transcripts in Tissue Sections
- In Situ Hybridization with 35S-Labeled Probes for Retinoid Receptors
- Isolation of Retinoid Receptors from Manimalian Cells
- Analysis of Retinoid Receptor Phosphorylation
- Photoaffinity Labeling of RARs and Mapping of Labeled Sites by an Endoproteinase Combination Technique
- PCR Cloning of N-Terminal RAR Isoforms and APL-Associated PLZF-RAR? Fusion Proteins
- RT-PCR in Diagnosis and Disease Monitoring of Acute Promyelocytic Leukemia (APL)
- A Two-Hybrid Protein Interaction System to Identify Factors That Interact with Retinoid and Vitamin D Receptors
- Gel-Shift Analysis and Identification of RXREs and RAREs by PCR-Based Selection
- Identification and Cloning of RA-Regulated Genes by mRNA-Differential Display
- Gene Targeting of Retinoid Receptors.