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04147nam a22003015i 4500 |
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978-1-59259-580-8 |
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20190618051914.0 |
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cr nn 008mamaa |
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100301s1998 xxu| s |||| 0|eng d |
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|a 9781592595808
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| 024 |
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|a 10.1385/0896035190
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Cytochrome P450 Protocols
|c edited by Ian R. Phillips, Elizabeth A. Shephard.
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| 250 |
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|a 1st ed. 1998.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1998.
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| 300 |
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|a XIV, 482 p. 49 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 107
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| 505 |
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|a The Role of Methods in the Discovery of the Cytochromes P450 -- Cytochrome P450 Nomenclature -- Spectral Analyses of Cytochromes P450 -- Purification of Cytochromes P450 -- Isolation and Purification of Constitutive Forms of Microsomal Cytochrome P450 -- Purification of Extrahepatic Cytochromes P450 -- Purification of Cytochromes P450: Products of Bacterial Recombinant Expression Systems -- Cytochrome P450 Reconstitution Systems -- Catalytic Assays for Human Cytochrome P450 -- Enzymatic Analysis of cDNA-Expressed Human CYP1A1, CYP1A2, and CYP1B1 with 7-Ethoxyresorufin as Substrate -- Spectrofluorometric Analysis of CYP2A6-Catalyzed Coumarin 7-Hydroxylation -- Determination of the CYP2B6 Component of 7-Ethoxy-4-Trifluoromethylcoumarin O-Deethylation Activity in Human Liver Microsomes -- High-Performance Liquid Chromatographic Analysis of CYP2CSCatalyzed Paclitaxel 6?-Hydroxylation -- Determination of CYP2C9-Catalyzed Diclofenac 4?-Hydroxylation by High-Performance Liquid Chromatography -- CYP2Cl g-Mediated (S)-Mephenytoin 4’-Hydroxylation Assayed by High-Performance Liquid Chromatography with Radiometric Detection -- CYP2D6-Dependent Bufuralol 1’-Hydroxylation Assayed by Reversed-Phase lon-Pair High-Performance Liquid Chromatography with Fluorescence Detection -- Spectrophotometric Analysis of Human CYP2E1-Catalyzed p-Nitrophenol Hydroxylation -- Thin-Layer Chromatographic Analysis of Human CYP3A-Catalyzed Testosterone 6?-Hydroxylation -- Determination of CYP4A11-Catalyzed Lauric Acid 12-Hydroxylation by High-Performance Liquid Chromatography with Radiometric Detection -- An Isocratic High-Performance Liquid Chromatographic Assay for CYP7Al-Catalyzed Cholesterol 7?-Hyciroxylation -- Use of 7-Ethoxycoumarinto Monitor Multiple Enzymesin the Human CYP1, CYP2, and CYP3 Families -- Expression of Eukaryotic Cytochromes P450 in E. coli -- Expression of Mammalian Cytochromes P450 in Yeast -- Expression of Cytochromes P450 in a Baculovirus System -- Expression of Cytochromes P450 in Mammalian Cells -- Monoclonal Antibodies to Cytochromes P450 -- Targeting Antipeptide Antibodies Towards Cytochrome P450 Enzymes -- Signals for Retention of Cytochrome P450 in the Endoplasmic Reticulum -- Quantification of Cytochrome P450 Gene Expression by RNase Protection Analysis -- Hepatocyte Cultures in Drug Metabolism and Toxicological Research and Testing -- Isolation of Rat Hepatocytes -- Collagen-Gel Cultures of Rat Hepatocytes: -- Rat Hepatocyte Cultures -- Isolation of Periportal and Pericentral Hepatocytes -- Perifusion Culture of Hepatocytes -- Human Hepatocyte Culture -- Reconstruction of Human Epidermis In Vitro -- Gene Transfer and Expression -- Lipid-Mediated Gene Transfer into Normal Adult Human Hepatocytes in Primary Culture -- Identification of Regulatory Protein- Binding Sites in Cytochrome P450 Genes by Means of the Gel- Retardation Assay -- Cytochrome P450 Gene Regulation -- Analysis of Cytochrome P450 Polymorphisms -- Induction of Cytochrome P4501A (CYP1A) in Fish A Biomarker for Environmental Pollution -- Generation of Allelic Series in Mice by Gene Targeting.
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| 650 |
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|a Biochemistry.
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| 650 |
1 |
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|a Biochemistry, general.
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| 700 |
1 |
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|a Phillips, Ian R.
|e editor.
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| 700 |
1 |
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|a Shephard, Elizabeth A.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/0896035190
|