Cytochrome P450 Protocols
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1998.
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| Edición: | 1st ed. 1998. |
| Colección: | Methods in Molecular Biology,
107 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/0896035190 |
Tabla de Contenidos:
- The Role of Methods in the Discovery of the Cytochromes P450
- Cytochrome P450 Nomenclature
- Spectral Analyses of Cytochromes P450
- Purification of Cytochromes P450
- Isolation and Purification of Constitutive Forms of Microsomal Cytochrome P450
- Purification of Extrahepatic Cytochromes P450
- Purification of Cytochromes P450: Products of Bacterial Recombinant Expression Systems
- Cytochrome P450 Reconstitution Systems
- Catalytic Assays for Human Cytochrome P450
- Enzymatic Analysis of cDNA-Expressed Human CYP1A1, CYP1A2, and CYP1B1 with 7-Ethoxyresorufin as Substrate
- Spectrofluorometric Analysis of CYP2A6-Catalyzed Coumarin 7-Hydroxylation
- Determination of the CYP2B6 Component of 7-Ethoxy-4-Trifluoromethylcoumarin O-Deethylation Activity in Human Liver Microsomes
- High-Performance Liquid Chromatographic Analysis of CYP2CSCatalyzed Paclitaxel 6?-Hydroxylation
- Determination of CYP2C9-Catalyzed Diclofenac 4?-Hydroxylation by High-Performance Liquid Chromatography
- CYP2Cl g-Mediated (S)-Mephenytoin 4’-Hydroxylation Assayed by High-Performance Liquid Chromatography with Radiometric Detection
- CYP2D6-Dependent Bufuralol 1’-Hydroxylation Assayed by Reversed-Phase lon-Pair High-Performance Liquid Chromatography with Fluorescence Detection
- Spectrophotometric Analysis of Human CYP2E1-Catalyzed p-Nitrophenol Hydroxylation
- Thin-Layer Chromatographic Analysis of Human CYP3A-Catalyzed Testosterone 6?-Hydroxylation
- Determination of CYP4A11-Catalyzed Lauric Acid 12-Hydroxylation by High-Performance Liquid Chromatography with Radiometric Detection
- An Isocratic High-Performance Liquid Chromatographic Assay for CYP7Al-Catalyzed Cholesterol 7?-Hyciroxylation
- Use of 7-Ethoxycoumarinto Monitor Multiple Enzymesin the Human CYP1, CYP2, and CYP3 Families
- Expression of Eukaryotic Cytochromes P450 in E. coli
- Expression of Mammalian Cytochromes P450 in Yeast
- Expression of Cytochromes P450 in a Baculovirus System
- Expression of Cytochromes P450 in Mammalian Cells
- Monoclonal Antibodies to Cytochromes P450
- Targeting Antipeptide Antibodies Towards Cytochrome P450 Enzymes
- Signals for Retention of Cytochrome P450 in the Endoplasmic Reticulum
- Quantification of Cytochrome P450 Gene Expression by RNase Protection Analysis
- Hepatocyte Cultures in Drug Metabolism and Toxicological Research and Testing
- Isolation of Rat Hepatocytes
- Collagen-Gel Cultures of Rat Hepatocytes:
- Rat Hepatocyte Cultures
- Isolation of Periportal and Pericentral Hepatocytes
- Perifusion Culture of Hepatocytes
- Human Hepatocyte Culture
- Reconstruction of Human Epidermis In Vitro
- Gene Transfer and Expression
- Lipid-Mediated Gene Transfer into Normal Adult Human Hepatocytes in Primary Culture
- Identification of Regulatory Protein- Binding Sites in Cytochrome P450 Genes by Means of the Gel- Retardation Assay
- Cytochrome P450 Gene Regulation
- Analysis of Cytochrome P450 Polymorphisms
- Induction of Cytochrome P4501A (CYP1A) in Fish A Biomarker for Environmental Pollution
- Generation of Allelic Series in Mice by Gene Targeting.