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03282nam a22002895i 4500 |
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978-1-59259-581-5 |
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20190618061151.0 |
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cr nn 008mamaa |
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100301s1999 xxu| s |||| 0|eng d |
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|a 9781592595815
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7 |
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|a 10.1385/1592595812
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Lipase and Phospholipase Protocols
|c edited by Mark Doolittle.
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| 250 |
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|a 1st ed. 1999.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1999.
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| 300 |
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|a XVII, 362 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 109
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|a Lipase Activity, Purification, and Expression -- Phospholipase A2 and Phosphatidylinositol-Specific Phospholipase C Assays by HPLC and TLC with Fluorescent Substrate -- Fluorometric Phospholipase Assays Based on Polymerized Liposome Substrates -- Triglyceride Lipase Assays Based on a Novel Fluorogenic Alkyldiacyl Glycerol Substrate -- Purification and Assay of Mammalian Group I and Group IIa Secretory Phospholipase A2 -- Determination of Plasmalogen-Selective Phospholipase A2 Activity by Radiochemical and Fluorometric Assay Procedures -- Human Plasma Platelet-Activating Factor Acetylhydrolase -- Assays for Pancreatic Triglyceride Lipase and Colipase -- Bile Salt-Activated Lipase -- Determining Lipoprotein Lipase and Hepatic Lipase Activity Using Radiolabeled Substrates -- Lysosomal Acid Lipase -- Hormone-Sensitive Lipase and Neutral Cholesteryl Ester Lipase -- Lecithin-Cholesterol Acyltransferase -- Purification of Lipases and Phospholipases by Heparin-Sepharose Chromatography -- Large-Scale Lipoprotein Lipase Purification from Adipose Tissue -- Purification of Rat Hepatic Lipase Essentially Free of Apolipoprotein E and Apolipoprotein B -- High-Level Expression and Purification of Human Hepatic Lipase from Mammalian Cells -- High-Level Baculoviral Expression of Hormone-Sensitive Lipase -- High-Level Baculoviral Expression of Lysosomal Acid Lipase -- One-Step Purification and Biochemical Characterization of Recombinant Pancreatic Lipases Expressed in Insect Cells -- Modulation of the Expression Level of Human Acidic Lipases by Various Signal Peptides -- Biochemical Characterization -- Immunodetection of Lipoprotein Lipase: Antibody Production, Immunoprecipitation, and Western Blotting Techniques -- Immunological Characterization of Digestive Lipases -- Determining Lipase Subunit Structure by Sucrose Gradient Centrifugation -- Detecting Ligands Interacting with Lipoprotein Lipase -- Monolayer Techniques for Studying Lipase Kinetics -- Efficient Immobilization of Phospholipase A2 -- Molecular Genetic Characterization -- An Introduction to Internet Resources for the Molecular and Genetic Analysis of the Lipases -- Techniques for the Measurement of Lipoprotein Lipase Messenger RNA -- In Vitro Transcription and Translation of Lipoprotein Lipase -- Induced Lipase Mutations in the Mouse.
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| 650 |
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|a Biochemistry.
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| 650 |
1 |
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|a Biochemistry, general.
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| 700 |
1 |
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|a Doolittle, Mark.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/1592595812
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