Lipase and Phospholipase Protocols
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1999.
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| Edición: | 1st ed. 1999. |
| Colección: | Methods in Molecular Biology,
109 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/1592595812 |
Tabla de Contenidos:
- Lipase Activity, Purification, and Expression
- Phospholipase A2 and Phosphatidylinositol-Specific Phospholipase C Assays by HPLC and TLC with Fluorescent Substrate
- Fluorometric Phospholipase Assays Based on Polymerized Liposome Substrates
- Triglyceride Lipase Assays Based on a Novel Fluorogenic Alkyldiacyl Glycerol Substrate
- Purification and Assay of Mammalian Group I and Group IIa Secretory Phospholipase A2
- Determination of Plasmalogen-Selective Phospholipase A2 Activity by Radiochemical and Fluorometric Assay Procedures
- Human Plasma Platelet-Activating Factor Acetylhydrolase
- Assays for Pancreatic Triglyceride Lipase and Colipase
- Bile Salt-Activated Lipase
- Determining Lipoprotein Lipase and Hepatic Lipase Activity Using Radiolabeled Substrates
- Lysosomal Acid Lipase
- Hormone-Sensitive Lipase and Neutral Cholesteryl Ester Lipase
- Lecithin-Cholesterol Acyltransferase
- Purification of Lipases and Phospholipases by Heparin-Sepharose Chromatography
- Large-Scale Lipoprotein Lipase Purification from Adipose Tissue
- Purification of Rat Hepatic Lipase Essentially Free of Apolipoprotein E and Apolipoprotein B
- High-Level Expression and Purification of Human Hepatic Lipase from Mammalian Cells
- High-Level Baculoviral Expression of Hormone-Sensitive Lipase
- High-Level Baculoviral Expression of Lysosomal Acid Lipase
- One-Step Purification and Biochemical Characterization of Recombinant Pancreatic Lipases Expressed in Insect Cells
- Modulation of the Expression Level of Human Acidic Lipases by Various Signal Peptides
- Biochemical Characterization
- Immunodetection of Lipoprotein Lipase: Antibody Production, Immunoprecipitation, and Western Blotting Techniques
- Immunological Characterization of Digestive Lipases
- Determining Lipase Subunit Structure by Sucrose Gradient Centrifugation
- Detecting Ligands Interacting with Lipoprotein Lipase
- Monolayer Techniques for Studying Lipase Kinetics
- Efficient Immobilization of Phospholipase A2
- Molecular Genetic Characterization
- An Introduction to Internet Resources for the Molecular and Genetic Analysis of the Lipases
- Techniques for the Measurement of Lipoprotein Lipase Messenger RNA
- In Vitro Transcription and Translation of Lipoprotein Lipase
- Induced Lipase Mutations in the Mouse.