Herpes Simplex Virus Protocols
Corporate Author: | |
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Other Authors: | , |
Format: | eBook |
Language: | English |
Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1998.
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Edition: | 1st ed. 1998. |
Series: | Methods in Molecular Medicine,
10 |
Subjects: | |
Online Access: | https://doi.org/10.1385/0896033473 |
Table of Contents:
- HSV Growth, Preparation, and Assay
- HSV Entry and Spread
- Preparation of HSV-DNA and Production of Infectious Virus
- HSV Mutagenesis
- Saturating Mutagenesis and Characterization of a Herpesvirus Genome Using In Vivo Reconstitution of Virus from Cloned Subgenomic Regions
- Construction and Use of Cell Lines Expressing HSV Genes
- Analysis of HSV Polypeptides
- Protein Purification
- Expression and Purification of Secreted Forms of HSV Glycoproteins from Baculovirus-Infected Insect Cells
- Crystallization of Macromolecules for Three-Dimensional Structure Determination
- Direct Immunogold Labeling of Herpesvirus Suspensions
- Expression of HSV Proteins in Bacteria
- In Vitro Systems to Analyze HSV Transcript Processing
- Analysis of HSV-1 Transcripts by RNA-PCR
- Transient Assays for HSV Origin and Replication Protein Function
- HSV Amplicons in Gene Therapy
- Analyses of HSV Proteins for Posttranslational Modifications and Enzyme Functions
- HSV-Cellular Protein Interactions
- Models of Recurrent Infection with HSV in the Skin and Eye of the Mouse
- Pathogenesis and Molecular Biology of HSV Latency and Ocular Reactivation in the Rabbit
- HSV Latency In Vitro
- Assessing Cell-Mediated Immune Responses to HSV in Murine Systems
- Assays for HSV Gene Expression During Establishment and Maintenance of Latent Infection
- Analysis of HSV-DNA and RNA Using the Polymerase Chain Reaction
- HSV Vectors for Gene Therapy
- Investigation of the Anti-HSV Activity of Candidate Antiviral Agents.