Herpes Simplex Virus Protocols

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Brown, S. Moira. (Editor), MacLean, Alasdair R. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 1998.
Edition:1st ed. 1998.
Series:Methods in Molecular Medicine, 10
Subjects:
Online Access:https://doi.org/10.1385/0896033473
Table of Contents:
  • HSV Growth, Preparation, and Assay
  • HSV Entry and Spread
  • Preparation of HSV-DNA and Production of Infectious Virus
  • HSV Mutagenesis
  • Saturating Mutagenesis and Characterization of a Herpesvirus Genome Using In Vivo Reconstitution of Virus from Cloned Subgenomic Regions
  • Construction and Use of Cell Lines Expressing HSV Genes
  • Analysis of HSV Polypeptides
  • Protein Purification
  • Expression and Purification of Secreted Forms of HSV Glycoproteins from Baculovirus-Infected Insect Cells
  • Crystallization of Macromolecules for Three-Dimensional Structure Determination
  • Direct Immunogold Labeling of Herpesvirus Suspensions
  • Expression of HSV Proteins in Bacteria
  • In Vitro Systems to Analyze HSV Transcript Processing
  • Analysis of HSV-1 Transcripts by RNA-PCR
  • Transient Assays for HSV Origin and Replication Protein Function
  • HSV Amplicons in Gene Therapy
  • Analyses of HSV Proteins for Posttranslational Modifications and Enzyme Functions
  • HSV-Cellular Protein Interactions
  • Models of Recurrent Infection with HSV in the Skin and Eye of the Mouse
  • Pathogenesis and Molecular Biology of HSV Latency and Ocular Reactivation in the Rabbit
  • HSV Latency In Vitro
  • Assessing Cell-Mediated Immune Responses to HSV in Murine Systems
  • Assays for HSV Gene Expression During Establishment and Maintenance of Latent Infection
  • Analysis of HSV-DNA and RNA Using the Polymerase Chain Reaction
  • HSV Vectors for Gene Therapy
  • Investigation of the Anti-HSV Activity of Candidate Antiviral Agents.