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04095nam a22003015i 4500 |
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978-1-59259-601-0 |
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20190618111835.0 |
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cr nn 008mamaa |
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100301s1999 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592596010
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| 024 |
7 |
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|a 10.1385/0896033694
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a HIV Protocols
|c edited by Nelson Michael, Jerome H. Kim.
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| 250 |
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|a 1st ed. 1999.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1999.
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| 300 |
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|a XIII, 421 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Medicine,
|v 17
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| 505 |
0 |
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|a Virology -- Isolation and Expansion of HIV from Cells and Body Fluids by Coculture -- Quantitative HIV Culture -- Quantitation of Cell-Free HIV by Reverse Transcriptase Activity -- Determination of Syncytium-Inducing Phenotype of Primary HIV-1 Isolates Using MT-2 cells -- Determination of HIV-1 Chemokine Coreceptor Tropism Using Transduced Human Osteosarcoma (HOS) Cells -- Use of Luciferase Reporter Viruses for Studying HIV Entry -- A Cell-Cell Fusion Assay to Monitor HIV-1 Env Interactions with Chemokine Receptors -- Methods of Culturing HIV-1 from Semen -- Molecular Biology -- Detection of HIV-1 Nucleic Acids by Southern Blotting -- Detection of HIV-1 Nucleic Acids by Northern Blotting -- Rapid Identification of Cloned HIV-1 Fragments -- PCR Amplification and Cloning of Virtually Full-Length HIV-1 Provirus -- Quantitation of HIV-1gag DNA and RNA From Single Frozen Cell Pellets -- Quantitative PCR for HIV-1 Proviral DNA -- Diagnosis and Direct Automated Sequencing of HIV-1 From Dried Blood Spots (DBS) Collected on Filter Paper -- Quantification of HIV-1 RNA in Dried Plasma Spots (DPS) -- Quantitation of HIV-1 RNA in Genital Secretions -- Detection of Nucleic Acids in Cells and Tissues by In Situ Polymerase Chain Reaction -- Determination of the Promoter Activity of HIV-1 Using the Chloramphenicol Acetyltransferase Reporter Gene Assay -- In Vitro Techniques for Studies of HIV-1 Promoter Activity -- Detection of Polymorphisms in the HIV-1 Coreceptor CCR5 Using Single-Strand Conformation Polymorphism -- Sequence-Specific Priming as a Rapid Screen for Known Mutations -- Quantitation of HIV-1 Entry Cofactor Expression -- Retrovirus-Mediated Gene Transduction of SupT1 Cells -- Cloning of HIV Single-Strand DNA Binding Protein from Human Lymphocyte Lambda gt11 Expression Library with [32P]-Oligomers -- Telomeric Terminal Restriction Fragment (TRF) -- Humoral Immunology -- Serologic Analysis by Enzyme-Linked Immunosorbent Assay (ELISA) -- Quantitative Immunoblotting with Fusion Proteins -- Analysis of Antibody Interactions with HIV-1 Envelope Expressed on the Surface of Acutely Infected H9 Cells -- Analysis of Antibody-Antigen Interactions Using Surface Plasmon Resonance -- Measurement of HIV-1 Specific and Total Antibody Secreting Cells by ELISPOT -- Linear Epitope Mapping by the PEPSCAN Method -- Neutralization of HIV-1 Infection of Human Peripheral Blood Mononuclear Cells (PBMC) -- Neutralization of HIV-1 Infection of Human Peripheral Blood Mononuclear Cells (PBMC) -- Collection and Processing of Mucosal Secretions from HIV-1 Infected Women -- Collection and Processing of Mucosal Secretions from Mice -- Cellular Immunology -- Lymphocyte Proliferation Assay -- HIV-1-Specific Cytotoxic T-Cell Assays -- HIV-1-Specific Antibody-Dependent Cellular Cytotoxicity (ADCC) -- Evaluation of Natural Killer Cell Activity -- Generation and Expansion of HIV-1 CD4+ Antigen-Specific T Cell Lines -- Immunophenotyping and Assessment of Cell Function by Three-Color Flow Cytometry of Peripheral Blood Lymphocytes -- Detection of Apoptosis in HIV-Infected Ceil Populations using TUNEL.
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| 650 |
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|a Immunology.
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| 650 |
1 |
4 |
|a Immunology.
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| 700 |
1 |
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|a Michael, Nelson.
|e editor.
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| 700 |
1 |
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|a Kim, Jerome H.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/0896033694
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