HIV Protocols
| Autor Corporativo: | |
|---|---|
| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1999.
|
| Edición: | 1st ed. 1999. |
| Colección: | Methods in Molecular Medicine,
17 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/0896033694 |
Tabla de Contenidos:
- Virology
- Isolation and Expansion of HIV from Cells and Body Fluids by Coculture
- Quantitative HIV Culture
- Quantitation of Cell-Free HIV by Reverse Transcriptase Activity
- Determination of Syncytium-Inducing Phenotype of Primary HIV-1 Isolates Using MT-2 cells
- Determination of HIV-1 Chemokine Coreceptor Tropism Using Transduced Human Osteosarcoma (HOS) Cells
- Use of Luciferase Reporter Viruses for Studying HIV Entry
- A Cell-Cell Fusion Assay to Monitor HIV-1 Env Interactions with Chemokine Receptors
- Methods of Culturing HIV-1 from Semen
- Molecular Biology
- Detection of HIV-1 Nucleic Acids by Southern Blotting
- Detection of HIV-1 Nucleic Acids by Northern Blotting
- Rapid Identification of Cloned HIV-1 Fragments
- PCR Amplification and Cloning of Virtually Full-Length HIV-1 Provirus
- Quantitation of HIV-1gag DNA and RNA From Single Frozen Cell Pellets
- Quantitative PCR for HIV-1 Proviral DNA
- Diagnosis and Direct Automated Sequencing of HIV-1 From Dried Blood Spots (DBS) Collected on Filter Paper
- Quantification of HIV-1 RNA in Dried Plasma Spots (DPS)
- Quantitation of HIV-1 RNA in Genital Secretions
- Detection of Nucleic Acids in Cells and Tissues by In Situ Polymerase Chain Reaction
- Determination of the Promoter Activity of HIV-1 Using the Chloramphenicol Acetyltransferase Reporter Gene Assay
- In Vitro Techniques for Studies of HIV-1 Promoter Activity
- Detection of Polymorphisms in the HIV-1 Coreceptor CCR5 Using Single-Strand Conformation Polymorphism
- Sequence-Specific Priming as a Rapid Screen for Known Mutations
- Quantitation of HIV-1 Entry Cofactor Expression
- Retrovirus-Mediated Gene Transduction of SupT1 Cells
- Cloning of HIV Single-Strand DNA Binding Protein from Human Lymphocyte Lambda gt11 Expression Library with [32P]-Oligomers
- Telomeric Terminal Restriction Fragment (TRF)
- Humoral Immunology
- Serologic Analysis by Enzyme-Linked Immunosorbent Assay (ELISA)
- Quantitative Immunoblotting with Fusion Proteins
- Analysis of Antibody Interactions with HIV-1 Envelope Expressed on the Surface of Acutely Infected H9 Cells
- Analysis of Antibody-Antigen Interactions Using Surface Plasmon Resonance
- Measurement of HIV-1 Specific and Total Antibody Secreting Cells by ELISPOT
- Linear Epitope Mapping by the PEPSCAN Method
- Neutralization of HIV-1 Infection of Human Peripheral Blood Mononuclear Cells (PBMC)
- Neutralization of HIV-1 Infection of Human Peripheral Blood Mononuclear Cells (PBMC)
- Collection and Processing of Mucosal Secretions from HIV-1 Infected Women
- Collection and Processing of Mucosal Secretions from Mice
- Cellular Immunology
- Lymphocyte Proliferation Assay
- HIV-1-Specific Cytotoxic T-Cell Assays
- HIV-1-Specific Antibody-Dependent Cellular Cytotoxicity (ADCC)
- Evaluation of Natural Killer Cell Activity
- Generation and Expansion of HIV-1 CD4+ Antigen-Specific T Cell Lines
- Immunophenotyping and Assessment of Cell Function by Three-Color Flow Cytometry of Peripheral Blood Lymphocytes
- Detection of Apoptosis in HIV-Infected Ceil Populations using TUNEL.