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02602nam a22002895i 4500 |
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978-1-59259-642-3 |
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20191026202655.0 |
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cr nn 008mamaa |
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100301s1998 xxu| s |||| 0|eng d |
| 020 |
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|a 9781592596423
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| 024 |
7 |
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|a 10.1007/978-1-59259-642-3
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Molecular Biomethods Handbook
|c edited by Ralph Rapley, John M. Walker.
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| 250 |
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|a 1st ed. 1998.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 1998.
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| 300 |
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|a XIII, 725 p. 360 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 505 |
0 |
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|a Extraction of Total RNA from Tissues and Cultured Cells -- Isolation of Total Cellular DNA from Tissues and Cultured Cells -- Gel Electrophoresis of DNA -- Sl Nuclease Analysis of RNA -- Detecting mRNA by Use of the Ribonuclease Protection Assay (RPA) -- Gene Probes -- Nonradioactive Labeling of DNA -- Southern Blot Analysis -- Northern Blot Analysis -- DNA Sequencing -- Autoradiography and Fluorography -- Mobility Shift Assays -- cDNA Libraries -- Genomic DNA Libraries -- ? as a Cloning Vector -- Plasmid-Derived Cloning Vectors -- M13 and Phagemid-Based Cloning Vectors -- Retroviral Vectors From Laboratory Tools to Molecular Medicines -- Baculovirus Vectors -- Gene Transfer and Expression in Tissue Culture Cells of Higher Eukaryotes -- Plant Transformation -- Restriction Fragment-Length Polymorphisms -- Genome Mapping -- Yeast Artificial Chromosomes -- Polymerase Chain Reaction -- In Vitro Transcriptions -- In Vitro Translation -- Site-Directed Mutagenesis -- Site-Directed Mutagenesis -- DNA Profiling -- Radiolabeling of Peptides and Proteins -- Protein Electrophoresis -- Free Zone Capillary Electrophoresis -- Protein Blotting -- Ion-Exchange Chromatography -- Size-Exclusion Chromatography -- Hydrophobic Interaction Chromatography -- Affinity Chromatography -- Reversed-Phase HPLC -- Glycoprotein Analysis -- Protein Sequencing -- Solid-Phase Peptide Synthesis -- Protein Engineering -- Monoclonal Antibodies -- Phage-Display Libraries -- Enzyme- Linked Immunosorbent Assay (ELISA) -- Epitope Mapping -- lmmunocytochemistry -- Flow Cytometry -- Mass Spectrometry -- The Technique of In Situ Hybridization.
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| 650 |
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|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Rapley, Ralph.
|e editor.
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| 700 |
1 |
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|a Walker, John M.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
4 |
0 |
|u https://doi.org/10.1007/978-1-59259-642-3
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