Molecular Biomethods Handbook
| Corporate Author: | |
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| Other Authors: | , |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1998.
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| Edition: | 1st ed. 1998. |
| Subjects: | |
| Online Access: | https://doi.org/10.1007/978-1-59259-642-3 |
Table of Contents:
- Extraction of Total RNA from Tissues and Cultured Cells
- Isolation of Total Cellular DNA from Tissues and Cultured Cells
- Gel Electrophoresis of DNA
- Sl Nuclease Analysis of RNA
- Detecting mRNA by Use of the Ribonuclease Protection Assay (RPA)
- Gene Probes
- Nonradioactive Labeling of DNA
- Southern Blot Analysis
- Northern Blot Analysis
- DNA Sequencing
- Autoradiography and Fluorography
- Mobility Shift Assays
- cDNA Libraries
- Genomic DNA Libraries
- ? as a Cloning Vector
- Plasmid-Derived Cloning Vectors
- M13 and Phagemid-Based Cloning Vectors
- Retroviral Vectors From Laboratory Tools to Molecular Medicines
- Baculovirus Vectors
- Gene Transfer and Expression in Tissue Culture Cells of Higher Eukaryotes
- Plant Transformation
- Restriction Fragment-Length Polymorphisms
- Genome Mapping
- Yeast Artificial Chromosomes
- Polymerase Chain Reaction
- In Vitro Transcriptions
- In Vitro Translation
- Site-Directed Mutagenesis
- Site-Directed Mutagenesis
- DNA Profiling
- Radiolabeling of Peptides and Proteins
- Protein Electrophoresis
- Free Zone Capillary Electrophoresis
- Protein Blotting
- Ion-Exchange Chromatography
- Size-Exclusion Chromatography
- Hydrophobic Interaction Chromatography
- Affinity Chromatography
- Reversed-Phase HPLC
- Glycoprotein Analysis
- Protein Sequencing
- Solid-Phase Peptide Synthesis
- Protein Engineering
- Monoclonal Antibodies
- Phage-Display Libraries
- Enzyme- Linked Immunosorbent Assay (ELISA)
- Epitope Mapping
- lmmunocytochemistry
- Flow Cytometry
- Mass Spectrometry
- The Technique of In Situ Hybridization.