DNA Repair Protocols

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Henderson, Daryl S. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 1999.
Edición:1st ed. 1999.
Colección:Methods in Molecular Biology, 113
Materias:
Acceso en línea:https://doi.org/10.1385/1592596754
LEADER 05274nam a22003135i 4500
001 978-1-59259-675-1
005 20191027133307.0
007 cr nn 008mamaa
008 100301s1999 xxu| s |||| 0|eng d
020 |a 9781592596751 
024 7 |a 10.1385/1592596754  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a DNA Repair Protocols  |c edited by Daryl S. Henderson. 
250 |a 1st ed. 1999. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 1999. 
300 |a XXII, 642 p.  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 113 
505 0 |a Mutant Isolation and Gene Cloning -- Isolation of DNA Structure-Dependent Checkpoint Mutants in S. pombe -- Isolating Mutants of the Nematode Caenorhabditis elegans That Are Hypersensitive to DNA-Damaging Agents -- Isolating DNA Repair Mutants of Drosophila melanogaster -- Generation, Identification, and Characterization of Repair-Defective Mutants of Arabidopsis -- Screening for ?-Ray Hypersensitive Mutants of Arabidopsis -- Isolation of Mutagen-Sensitive Chinese Hamster Cell Lines by Replica Plating -- Strategies for Cloning Mammalian DNA Repair Genes -- Novel Complementation Assays for DNA Repair-Deficient Cells -- Recognition and Removal of Inappropriate or Damaged DNA Bases -- The Use of Electrophoretic Mobility Shift Assays to Study DNA Repair -- Mismatch Repair Assay -- Measurement of Activities of Cyclobutane-Pyrimidine-Dimer and (6-4)-Photoproduct Photolyases -- A Dot Blot Immunoassay for UV Photoproducts -- Measurement of UV Radiation-Induced DNA Damage Using Specific Antibodies -- Quantification of Photoproducts in Mammalian Cell DNA Using Radioimmunoassay -- Monitoring Removal of Cyclobutane Pyrimidine Dimers in Arabidopsis -- DNA Damage Quantitation by Alkaline Gel Electrophoresis -- The Comet Assay (Single-Cell Gel Test) -- Measuring the Formation and Repair of UV Photoproducts by Ligation-Mediated PCR -- PCR-Based Assays for Strand-Specific Measurement of DNA Damage and Repair I -- PCR-Based Assays for Strand-Specific Measurement of DNA Damage and Repair II -- Gene-Specific and Mitochondrial Repair of Oxidative DNA Damage -- Characterization of DNA Strand Cleavage by Enzymes That Act at Abasic Sites in DNA -- Base Excision Repair Assay Using Xenopus laevis Oocyte Extracts -- In Vitro Base Excision Repair Assay Using Mammalian Cell Extracts -- Nucleotide Excision Repair in Saccharomyces cerevisiae Whole-Cell Extracts -- In Vitro Excision Repair Assay in Schizosaccharomyces pombe -- Nucleotide Excision Repair Assay in Drosophila melanogaster Using Established Cell Lines -- Nucleotide Excision Repair in Nuclear Extracts from Xenopus Oocytes -- Assay for Nucleotide Excision Repair Protein Activity Using Fractionated Cell Extracts and UV-Damaged Plasmid DNA -- Dual-Incision Assays for Nucleotide Excision Repair Using DNA with a Lesion at a Specific Site -- DNA Strand Breakage and Repair -- In Vitro Chemiluminescence Assay to Measure Excision Repair in Cell Extracts -- Physical Monitoring of HO-Induced Homologous Recombination -- Use of P Element Transposons to Study DNA Double-Strand Break Repair in Drosophila melanogaster -- Analyzing Double-Strand Repair Events in Drosophila -- Expression of I-Sce I in Drosophila to Induce DNA Double-Strand Breaks -- Use of I-Sce I to Induce DNA Double-Strand Breaks in Nicotiana -- Chromosomal Double-Strand Breaks Introduced in Mammalian Cells by Expression of I-Sce I Endonuclease -- Induction of DNA Double-Strand Breaks by Electroporation of Restriction Enzymes into Mammalian Cells -- In Vitro Rejoining of Double-Strand Breaks in Genomic DNA -- Extrachromosomal Assay for DNA Double-Strand Break Repair -- Use of Gene Targeting to Study Recombination in Mammalian DNA Repair Mutants -- DNA Damage Tolerance Mechanisms and Regulatory Responses -- Measurement of Low-Frequency DNA Breaks Using Nucleoid Flow Cytometry -- Live Analysis of the Division Cycles in X-Irradiated Drosophila Embryos -- Inhibition of DNA Synthesis by Ionizing Radiation -- Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of DNA Replication -- Assays of Bypass Replication of Genotoxic Lesions in Mammalian Disease and Mutant Cell-Free Extracts -- Detection of Chromatin-Bound PCNA in Cultured Cells Following Exposure to DNA-Damaging Agents -- Induction of p53 Protein as a Marker for Ionizing Radiation Exposure In Vivo -- Activation of p53 Protein Function in Response to Cellular Irradiation -- Selective Extraction of Fragmented DNA from Apoptotic Cells for Analysis by Gel Electrophoresis and Identification of Apoptotic Cells by Flow Cytometry -- Detection of DNA Strand Breakage in the Analysis of Apoptosis and Cell Proliferation by Flow and Laser Scanning Cytometry -- Immunoassay for Single-Stranded DNA in Apoptotic Cells. 
650 0 |a Biochemistry. 
650 0 |a Cell biology. 
650 1 4 |a Biochemistry, general. 
650 2 4 |a Cell Biology. 
700 1 |a Henderson, Daryl S.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
856 4 0 |u https://doi.org/10.1385/1592596754