DNA Repair Protocols

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Henderson, Daryl S. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 1999.
Edition:1st ed. 1999.
Series:Methods in Molecular Biology, 113
Subjects:
Online Access:https://doi.org/10.1385/1592596754
Table of Contents:
  • Mutant Isolation and Gene Cloning
  • Isolation of DNA Structure-Dependent Checkpoint Mutants in S. pombe
  • Isolating Mutants of the Nematode Caenorhabditis elegans That Are Hypersensitive to DNA-Damaging Agents
  • Isolating DNA Repair Mutants of Drosophila melanogaster
  • Generation, Identification, and Characterization of Repair-Defective Mutants of Arabidopsis
  • Screening for ?-Ray Hypersensitive Mutants of Arabidopsis
  • Isolation of Mutagen-Sensitive Chinese Hamster Cell Lines by Replica Plating
  • Strategies for Cloning Mammalian DNA Repair Genes
  • Novel Complementation Assays for DNA Repair-Deficient Cells
  • Recognition and Removal of Inappropriate or Damaged DNA Bases
  • The Use of Electrophoretic Mobility Shift Assays to Study DNA Repair
  • Mismatch Repair Assay
  • Measurement of Activities of Cyclobutane-Pyrimidine-Dimer and (6-4)-Photoproduct Photolyases
  • A Dot Blot Immunoassay for UV Photoproducts
  • Measurement of UV Radiation-Induced DNA Damage Using Specific Antibodies
  • Quantification of Photoproducts in Mammalian Cell DNA Using Radioimmunoassay
  • Monitoring Removal of Cyclobutane Pyrimidine Dimers in Arabidopsis
  • DNA Damage Quantitation by Alkaline Gel Electrophoresis
  • The Comet Assay (Single-Cell Gel Test)
  • Measuring the Formation and Repair of UV Photoproducts by Ligation-Mediated PCR
  • PCR-Based Assays for Strand-Specific Measurement of DNA Damage and Repair I
  • PCR-Based Assays for Strand-Specific Measurement of DNA Damage and Repair II
  • Gene-Specific and Mitochondrial Repair of Oxidative DNA Damage
  • Characterization of DNA Strand Cleavage by Enzymes That Act at Abasic Sites in DNA
  • Base Excision Repair Assay Using Xenopus laevis Oocyte Extracts
  • In Vitro Base Excision Repair Assay Using Mammalian Cell Extracts
  • Nucleotide Excision Repair in Saccharomyces cerevisiae Whole-Cell Extracts
  • In Vitro Excision Repair Assay in Schizosaccharomyces pombe
  • Nucleotide Excision Repair Assay in Drosophila melanogaster Using Established Cell Lines
  • Nucleotide Excision Repair in Nuclear Extracts from Xenopus Oocytes
  • Assay for Nucleotide Excision Repair Protein Activity Using Fractionated Cell Extracts and UV-Damaged Plasmid DNA
  • Dual-Incision Assays for Nucleotide Excision Repair Using DNA with a Lesion at a Specific Site
  • DNA Strand Breakage and Repair
  • In Vitro Chemiluminescence Assay to Measure Excision Repair in Cell Extracts
  • Physical Monitoring of HO-Induced Homologous Recombination
  • Use of P Element Transposons to Study DNA Double-Strand Break Repair in Drosophila melanogaster
  • Analyzing Double-Strand Repair Events in Drosophila
  • Expression of I-Sce I in Drosophila to Induce DNA Double-Strand Breaks
  • Use of I-Sce I to Induce DNA Double-Strand Breaks in Nicotiana
  • Chromosomal Double-Strand Breaks Introduced in Mammalian Cells by Expression of I-Sce I Endonuclease
  • Induction of DNA Double-Strand Breaks by Electroporation of Restriction Enzymes into Mammalian Cells
  • In Vitro Rejoining of Double-Strand Breaks in Genomic DNA
  • Extrachromosomal Assay for DNA Double-Strand Break Repair
  • Use of Gene Targeting to Study Recombination in Mammalian DNA Repair Mutants
  • DNA Damage Tolerance Mechanisms and Regulatory Responses
  • Measurement of Low-Frequency DNA Breaks Using Nucleoid Flow Cytometry
  • Live Analysis of the Division Cycles in X-Irradiated Drosophila Embryos
  • Inhibition of DNA Synthesis by Ionizing Radiation
  • Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of DNA Replication
  • Assays of Bypass Replication of Genotoxic Lesions in Mammalian Disease and Mutant Cell-Free Extracts
  • Detection of Chromatin-Bound PCNA in Cultured Cells Following Exposure to DNA-Damaging Agents
  • Induction of p53 Protein as a Marker for Ionizing Radiation Exposure In Vivo
  • Activation of p53 Protein Function in Response to Cellular Irradiation
  • Selective Extraction of Fragmented DNA from Apoptotic Cells for Analysis by Gel Electrophoresis and Identification of Apoptotic Cells by Flow Cytometry
  • Detection of DNA Strand Breakage in the Analysis of Apoptosis and Cell Proliferation by Flow and Laser Scanning Cytometry
  • Immunoassay for Single-Stranded DNA in Apoptotic Cells.