DNA Repair Protocols
| Corporate Author: | |
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
1999.
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| Edition: | 1st ed. 1999. |
| Series: | Methods in Molecular Biology,
113 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/1592596754 |
Table of Contents:
- Mutant Isolation and Gene Cloning
- Isolation of DNA Structure-Dependent Checkpoint Mutants in S. pombe
- Isolating Mutants of the Nematode Caenorhabditis elegans That Are Hypersensitive to DNA-Damaging Agents
- Isolating DNA Repair Mutants of Drosophila melanogaster
- Generation, Identification, and Characterization of Repair-Defective Mutants of Arabidopsis
- Screening for ?-Ray Hypersensitive Mutants of Arabidopsis
- Isolation of Mutagen-Sensitive Chinese Hamster Cell Lines by Replica Plating
- Strategies for Cloning Mammalian DNA Repair Genes
- Novel Complementation Assays for DNA Repair-Deficient Cells
- Recognition and Removal of Inappropriate or Damaged DNA Bases
- The Use of Electrophoretic Mobility Shift Assays to Study DNA Repair
- Mismatch Repair Assay
- Measurement of Activities of Cyclobutane-Pyrimidine-Dimer and (6-4)-Photoproduct Photolyases
- A Dot Blot Immunoassay for UV Photoproducts
- Measurement of UV Radiation-Induced DNA Damage Using Specific Antibodies
- Quantification of Photoproducts in Mammalian Cell DNA Using Radioimmunoassay
- Monitoring Removal of Cyclobutane Pyrimidine Dimers in Arabidopsis
- DNA Damage Quantitation by Alkaline Gel Electrophoresis
- The Comet Assay (Single-Cell Gel Test)
- Measuring the Formation and Repair of UV Photoproducts by Ligation-Mediated PCR
- PCR-Based Assays for Strand-Specific Measurement of DNA Damage and Repair I
- PCR-Based Assays for Strand-Specific Measurement of DNA Damage and Repair II
- Gene-Specific and Mitochondrial Repair of Oxidative DNA Damage
- Characterization of DNA Strand Cleavage by Enzymes That Act at Abasic Sites in DNA
- Base Excision Repair Assay Using Xenopus laevis Oocyte Extracts
- In Vitro Base Excision Repair Assay Using Mammalian Cell Extracts
- Nucleotide Excision Repair in Saccharomyces cerevisiae Whole-Cell Extracts
- In Vitro Excision Repair Assay in Schizosaccharomyces pombe
- Nucleotide Excision Repair Assay in Drosophila melanogaster Using Established Cell Lines
- Nucleotide Excision Repair in Nuclear Extracts from Xenopus Oocytes
- Assay for Nucleotide Excision Repair Protein Activity Using Fractionated Cell Extracts and UV-Damaged Plasmid DNA
- Dual-Incision Assays for Nucleotide Excision Repair Using DNA with a Lesion at a Specific Site
- DNA Strand Breakage and Repair
- In Vitro Chemiluminescence Assay to Measure Excision Repair in Cell Extracts
- Physical Monitoring of HO-Induced Homologous Recombination
- Use of P Element Transposons to Study DNA Double-Strand Break Repair in Drosophila melanogaster
- Analyzing Double-Strand Repair Events in Drosophila
- Expression of I-Sce I in Drosophila to Induce DNA Double-Strand Breaks
- Use of I-Sce I to Induce DNA Double-Strand Breaks in Nicotiana
- Chromosomal Double-Strand Breaks Introduced in Mammalian Cells by Expression of I-Sce I Endonuclease
- Induction of DNA Double-Strand Breaks by Electroporation of Restriction Enzymes into Mammalian Cells
- In Vitro Rejoining of Double-Strand Breaks in Genomic DNA
- Extrachromosomal Assay for DNA Double-Strand Break Repair
- Use of Gene Targeting to Study Recombination in Mammalian DNA Repair Mutants
- DNA Damage Tolerance Mechanisms and Regulatory Responses
- Measurement of Low-Frequency DNA Breaks Using Nucleoid Flow Cytometry
- Live Analysis of the Division Cycles in X-Irradiated Drosophila Embryos
- Inhibition of DNA Synthesis by Ionizing Radiation
- Analysis of Inhibition of DNA Replication in Irradiated Cells Using the SV40-Based In Vitro Assay of DNA Replication
- Assays of Bypass Replication of Genotoxic Lesions in Mammalian Disease and Mutant Cell-Free Extracts
- Detection of Chromatin-Bound PCNA in Cultured Cells Following Exposure to DNA-Damaging Agents
- Induction of p53 Protein as a Marker for Ionizing Radiation Exposure In Vivo
- Activation of p53 Protein Function in Response to Cellular Irradiation
- Selective Extraction of Fragmented DNA from Apoptotic Cells for Analysis by Gel Electrophoresis and Identification of Apoptotic Cells by Flow Cytometry
- Detection of DNA Strand Breakage in the Analysis of Apoptosis and Cell Proliferation by Flow and Laser Scanning Cytometry
- Immunoassay for Single-Stranded DNA in Apoptotic Cells.