Receptor Signal Transduction Protocols
| Corporate Author: | |
|---|---|
| Other Authors: | , |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2004.
|
| Edition: | 2nd ed. 2004. |
| Series: | Methods in Molecular Biology,
259 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/1592597548 |
Table of Contents:
- Radioligand-Binding Methods for Membrane Preparations and Intact Cells
- Determination of Allosteric Interactions Using Radioligand-Binding Techniques
- Generation, Use, and Validation of Receptor-Selective Antibodies
- Immunocytochemical Identification of G-Protein-Coupled Receptor Expression and Localization
- Generation and Use of Epitope-Tagged Receptors
- Identification of G-Protein-Coupled Receptor mRNA Expression by Northern Blotting and In Situ Hybridization
- Electron Microscopic Studies of Receptor Localization
- Generation of Model Cell Lines Expressing Recombinant G-Protein-Coupled Receptors
- Viral Infection Protocols
- Expression of G-Protein Signaling Components in Adult Mammalian Neurons by Microinjection
- Covalent Modification of G-Proteins by Affinity Labeling
- Measurement of Agonist-Stimulated [35S]GTP?S Binding to Assess Total G-Protein and G?-Subtype-Specific Activation by G-Protein-Coupled Receptors
- Identification and Quantitation of G-Protein ?-Subunits
- Analysis of Function of Receptor-G-Protein and Receptor-RGS Fusion Proteins
- Assessment of Receptor Internalization and Recycling
- G-Protein-Coupled Receptor Phosphorylation and Palmitoylation
- Identification of G-Protein-Coupled Receptor Phosphorylation Sites by 2D Phosphopeptide Mapping
- Ubiquitination of G-Protein-Coupled Receptors
- Receptor Mutagenesis Strategies for Examination of Structure-Function Relationships
- Study of G-Protein-Coupled Receptor-Protein Interactions by Bioluminescence Resonance Energy Transfer
- Fluorescence Resonance Energy Transfer to Study Receptor Dimerization in Living Cells
- Identification of Protein Interactions by Yeast Two-Hybrid Screening and Coimmunoprecipitation
- Study of G-Protein-Coupled Receptor-Protein Interactions Using Gel Overlay Assays and Glutathione-S-Transferase-Fusion Protein Pull-Downs
- Receptor Knock-Out and Knock-In Strategies
- Statistical Methods in G-Protein-Coupled Receptor Research.