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03004nam a22003255i 4500 |
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978-1-59259-827-4 |
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20191026221249.0 |
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cr nn 008mamaa |
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100301s2004 xxu| s |||| 0|eng d |
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|a 9781592598274
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7 |
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|a 10.1385/1592598277
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Transgenic Plants
|b Methods and Protocols /
|c edited by Leandro Peña.
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| 250 |
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|a 1st ed. 2004.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2004.
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| 300 |
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|a XIV, 437 p.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 286
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|a Transgenic Plants: An Historical Perspective -- Transformation -- Plant Transformation: Agrobacterium-Mediated Gene Transfer -- Production of Hairy Root Cultures and Transgenic Plants by Agrobacterium rhizogenes-Mediated Transformation -- Stable Transformation of Plant Cells by Particle Bombardment/Biolistics -- Electroporation: Introduction and Expression of Transgenes in Plant Protoplasts -- Floral Dip: Agrobacterium-Mediated Germ Line Transformation -- Production of Transgenic Crops by the Floral-Dip Method -- Chloroplast Genetic Engineering to Improve Agronomic Traits -- Regeneration -- Organogenesis From Transformed Tomato Explants -- Genetic Transformation of Conifers Utilizing Somatic Embryogenesis -- Regeneration of Transgenic Cassava From Transformed Embryogenic Tissues -- Genetic Transformation of Mature Citrus Plants -- Selection -- Selectable Markers: Antibiotic and Herbicide Resistance -- Histochemical and Fluorometric Assays for uidA (GUS) Gene Detection -- Green Fluorescent Protein Quantification in Whole Plants -- Positive Selection -- Elimination of Marker Genes From Transgenic Plants Using MAT Vector Systems -- Simple and Efficient Removal of Marker Genes From Plastids by Homologous Recombination -- Transgene Integration, Expression, and Localization -- The Study of Transgene Copy Number and Organization -- Analysis of Gene Expression in Transgenic Plants -- Transgene Integration: Use of Matrix Attachment Regions -- Fluorescence In Situ Hybridization to Localize Transgenes in Plant Chromosomes -- Amplification of Genomic Sequences Flanking T-DNA Insertions by Thermal Asymmetric Interlaced Polymerase Chain Reaction -- Risk Assessment -- Agrobacterium Persistence in Plant Tissues After Transformation -- Transgene Dispersal Through Pollen -- Transgenic Crops -- Transgenic Crops: The Current and Next Generations -- Comparative Development and Impact of Transgenic Papayas in Hawaii, Jamaica, and Venezuela.
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| 650 |
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|a Plant science.
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| 650 |
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|a Botany.
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| 650 |
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|a Transgenic organisms.
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| 650 |
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|a Plant Sciences.
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| 650 |
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4 |
|a Transgenics.
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| 700 |
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|a Peña, Leandro.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/1592598277
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