Transgenic Plants Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Peña, Leandro. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2004.
Edición:1st ed. 2004.
Colección:Methods in Molecular Biology, 286
Materias:
Acceso en línea:https://doi.org/10.1385/1592598277
Tabla de Contenidos:
  • Transgenic Plants: An Historical Perspective
  • Transformation
  • Plant Transformation: Agrobacterium-Mediated Gene Transfer
  • Production of Hairy Root Cultures and Transgenic Plants by Agrobacterium rhizogenes-Mediated Transformation
  • Stable Transformation of Plant Cells by Particle Bombardment/Biolistics
  • Electroporation: Introduction and Expression of Transgenes in Plant Protoplasts
  • Floral Dip: Agrobacterium-Mediated Germ Line Transformation
  • Production of Transgenic Crops by the Floral-Dip Method
  • Chloroplast Genetic Engineering to Improve Agronomic Traits
  • Regeneration
  • Organogenesis From Transformed Tomato Explants
  • Genetic Transformation of Conifers Utilizing Somatic Embryogenesis
  • Regeneration of Transgenic Cassava From Transformed Embryogenic Tissues
  • Genetic Transformation of Mature Citrus Plants
  • Selection
  • Selectable Markers: Antibiotic and Herbicide Resistance
  • Histochemical and Fluorometric Assays for uidA (GUS) Gene Detection
  • Green Fluorescent Protein Quantification in Whole Plants
  • Positive Selection
  • Elimination of Marker Genes From Transgenic Plants Using MAT Vector Systems
  • Simple and Efficient Removal of Marker Genes From Plastids by Homologous Recombination
  • Transgene Integration, Expression, and Localization
  • The Study of Transgene Copy Number and Organization
  • Analysis of Gene Expression in Transgenic Plants
  • Transgene Integration: Use of Matrix Attachment Regions
  • Fluorescence In Situ Hybridization to Localize Transgenes in Plant Chromosomes
  • Amplification of Genomic Sequences Flanking T-DNA Insertions by Thermal Asymmetric Interlaced Polymerase Chain Reaction
  • Risk Assessment
  • Agrobacterium Persistence in Plant Tissues After Transformation
  • Transgene Dispersal Through Pollen
  • Transgenic Crops
  • Transgenic Crops: The Current and Next Generations
  • Comparative Development and Impact of Transgenic Papayas in Hawaii, Jamaica, and Venezuela.