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02888nam a22002895i 4500 |
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978-1-59259-839-7 |
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20190618041043.0 |
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cr nn 008mamaa |
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100301s2005 xxu| s |||| 0|eng d |
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|a 9781592598397
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|a 10.1385/1592598390
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a Phosphodiesterase Methods and Protocols
|c edited by Claire Lugnier.
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|a 1st ed. 2005.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2005.
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| 300 |
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|a 336 p. 93 illus., 1 illus. in color.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 307
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|a Study of Cyclic Adenosine Monophosphate Microdomains in Cells -- High-Resolution Measurements of Cyclic Adenosine Monophosphate Signals in 3D Microdomains -- Cygnets -- High-Throughput Screening of Phosphodiesterase Activity in Living Cells -- Assessment of Phosphodiesterase Isozyme Contribution in Cell and Tissue Extracts -- Localization of the Cyclic Guanosine 3?,5?-Monophosphate- Hydrolyzing Phosphodiesterase Type 9 in Rat Brain by Nonradioactive In Situ Hybridization -- Determination of Ca2+/Calmodulin-Stimulated Phosphodiesterase Activity in Intact Cells -- Adenovirus-Mediated Overexpression of Murine Cyclic Nucleotide Phosphodiesterase 3B -- Identification of Promoter Elements in the 5?-Flanking Region of Murine Cyclic Nucleotide Phosphodiesterase 3B Gene -- Purification of PDE6 Isozymes From Mammalian Retina -- Cyclic Guanosine 5?-Monophosphate Binding to Regulatory GAF Domains of Photoreceptor Phosphodiesterase -- Renaturation of the Catalytic Domain of PDE4A Expressed in Escherichia coli as Inclusion Bodies -- Determining the Subunit Structure of Phosphodiesterases Using Gel Filtration and Sucrose Density Gradient Centrifugation -- Crystallization of Cyclic Nucleotide Phosphodiesterases -- Generation of PDE4 Knockout Mice by Gene Targeting -- Immunoprecipitation of PDE2 Phosphorylated and Inactivated by an Associated Protein Kinase -- Investigation of Extracellular Signal-Regulated Kinase 2 Mitogen-Activated Protein Kinase Phosphorylation and Regulation of Activity of PDE4 Cyclic Adenosine Monophosphate-Specific Phosphodiesterases -- Radiolabeled Ligand Binding to the Catalytic or Allosteric Sites of PDE5 and PDE11 -- Analysis of Dimerization Determinants of PDE6 Catalytic Subunits -- Interactions Between Catalytic and Inhibitory Subunits of PDE6 -- Purification, Reconstitution on Lipid Vesicles, and Assays of PDE6 and Its Activator G Protein, Transducin.
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| 650 |
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|a Biochemistry.
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|a Biochemistry, general.
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| 700 |
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|a Lugnier, Claire.
|e editor.
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| 710 |
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|a SpringerLink (Online service)
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|t Springer eBooks
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|u https://doi.org/10.1385/1592598390
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