Phosphodiesterase Methods and Protocols
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| Other Authors: | |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2005.
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| Edition: | 1st ed. 2005. |
| Series: | Methods in Molecular Biology,
307 |
| Subjects: | |
| Online Access: | https://doi.org/10.1385/1592598390 |
Table of Contents:
- Study of Cyclic Adenosine Monophosphate Microdomains in Cells
- High-Resolution Measurements of Cyclic Adenosine Monophosphate Signals in 3D Microdomains
- Cygnets
- High-Throughput Screening of Phosphodiesterase Activity in Living Cells
- Assessment of Phosphodiesterase Isozyme Contribution in Cell and Tissue Extracts
- Localization of the Cyclic Guanosine 3?,5?-Monophosphate- Hydrolyzing Phosphodiesterase Type 9 in Rat Brain by Nonradioactive In Situ Hybridization
- Determination of Ca2+/Calmodulin-Stimulated Phosphodiesterase Activity in Intact Cells
- Adenovirus-Mediated Overexpression of Murine Cyclic Nucleotide Phosphodiesterase 3B
- Identification of Promoter Elements in the 5?-Flanking Region of Murine Cyclic Nucleotide Phosphodiesterase 3B Gene
- Purification of PDE6 Isozymes From Mammalian Retina
- Cyclic Guanosine 5?-Monophosphate Binding to Regulatory GAF Domains of Photoreceptor Phosphodiesterase
- Renaturation of the Catalytic Domain of PDE4A Expressed in Escherichia coli as Inclusion Bodies
- Determining the Subunit Structure of Phosphodiesterases Using Gel Filtration and Sucrose Density Gradient Centrifugation
- Crystallization of Cyclic Nucleotide Phosphodiesterases
- Generation of PDE4 Knockout Mice by Gene Targeting
- Immunoprecipitation of PDE2 Phosphorylated and Inactivated by an Associated Protein Kinase
- Investigation of Extracellular Signal-Regulated Kinase 2 Mitogen-Activated Protein Kinase Phosphorylation and Regulation of Activity of PDE4 Cyclic Adenosine Monophosphate-Specific Phosphodiesterases
- Radiolabeled Ligand Binding to the Catalytic or Allosteric Sites of PDE5 and PDE11
- Analysis of Dimerization Determinants of PDE6 Catalytic Subunits
- Interactions Between Catalytic and Inhibitory Subunits of PDE6
- Purification, Reconstitution on Lipid Vesicles, and Assays of PDE6 and Its Activator G Protein, Transducin.