Molecular Toxicology Protocols

Molecular Toxicology Protocols

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Keohavong, Phouthone. (Editor ), Grant, Stephen G. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2005.
Edición:1st ed. 2005.
Colección:Methods in Molecular Biology, 291
Materias:
Pharmacology.
Biochemistry.
Pharmacology/Toxicology.
Biochemistry, general.
Acceso en línea:https://doi.org/10.1385/1592598404
Tabla de Contenidos:
  • Analysis of DNA Adducts
  • 32P-Postlabeling Analysis of DNA Adducts
  • Modification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single Spot
  • DNA Isolation and Sample Preparation for Quantification of Adduct Levels by Accelerator Mass Spectrometry
  • Fluoroimaging-Based Immunoassay of DNA Photoproducts in Ultraviolet-B-Irradiated Tadpoles
  • Analysis of DNA Strand Cleavage at Abasic Sites
  • Detection of Chromosomal and Genome-Wide Damage
  • Premature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes for Chromosome Aberration Analysis Using Specific Whole-Chromosome DNA Hybridization Probes
  • Mutagen-Induced Chromatid Breakage as a Marker of Cancer Risk
  • Flow Cytometric Analysis of Micronuclei in Erythrocytes
  • The Comet Assay
  • Computerized Image Analysis Software for the Comet Assay
  • The Comet-FISH Technique
  • DNA Double-Strand Break Damage and Repair Assessed by Pulsed-Field Gel Electrophoresis
  • Detection and Characterization of Surrogate Gene Mutation
  • Analysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse Lymphocytes
  • Quantifying In Vivo Somatic Mutations Using Transgenic Mouse Model Systems
  • Methods for Detecting Somatic Mutations In Vitro
  • Molecular Analysis of Mutations in the Human HPRT Gene
  • Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human Lymphocytes
  • The GPA In Vivo Somatic Mutation Assay
  • Flow Cytometric Measurement of Mutant T Cells With Altered Expression of TCR
  • Detection and Characterization of Cancer Gene Mutation
  • Mutation Screening of the TP53 Gene by Temporal Temperature Gradient Gel Electrophoresis
  • Analysis of K-RAS and P53 Mutations in Sputum Samples
  • Allele-Specific Competitive Blocker-PCR Detection of Rare Base Substitution
  • Gel-Based Nonradioactive Single-Strand Conformational Polymorphism and Mutation Detection
  • Detection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic Lesions
  • Detection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCR
  • Analysis of DNA Repair Mechanisms
  • Microsatellite Instability
  • Unscheduled DNA Synthesis
  • Analysis of DNA Repair Using Transfection-Based Host Cell Reactivation
  • An Immunoassay for Measuring Repair of Ultraviolet Photoproducts
  • Analysis of DNA Double-Strand Break Repair by Nonhomologous End Joining in Cell-Free Extracts From Mammalian Cells
  • Measuring Recombination Proficiency in Mouse Embryonic Stem Cells
  • Array Technologies
  • Strategies for Measurement of Biotransformation Enzyme Gene Expression
  • Genotyping Technologies
  • TaqMan® Fluorogenic Detection System to Analyze Gene Transcription in Autopsy Material
  • Development of Quantitative Reverse Transcriptase PCR Assays for Measuring Gene Expression
  • Apoptosis
  • Quantification of Selective Phosphatidylserine Oxidation During Apoptosis
  • Quantitative Method of Measuring Phosphatidylserine Externalization During Apoptosis Using Electron Paramagnetic Resonance Spectroscopy and Annexin-Conjugated Iron
  • Detection of Programmed Cell Death in Cells Exposed to Genotoxic Agents Using a Caspase Activation Assay.

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