Yeast Protocols
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2006.
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| Edición: | 2nd ed. 2006. |
| Colección: | Methods in Molecular Biology,
313 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/1592599583 |
Tabla de Contenidos:
- Basic Investigations in Saccharomyces cerevisiae
- Isolation of Nucleic Acids
- Purification of Yeast Peroxisomes
- Isolation of Yeast Plasma Membranes
- Isolation of Yeast Mitochondria
- Extraction of Yeast Lipids
- Two-Dimensional Gel Electrophoresis of Total Yeast Proteins
- Pulsed-Field Gel Electrophoresis of Budding Yeast Chromosomes
- Analysis of Yeast Lipids
- Yeast Fluorescence Microscopy
- Intracellular Expression of Recombinant Antibody Fluorescent Protein Fusions for Localization of Target Antigens in Schizosaccharomyces pombe
- Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method
- Mutagenesis
- Gene Disruption in the Budding Yeast Saccharomyces cerevisiae
- Inducible Degron and Its Application to Creating Conditional Mutants
- Synthetic Lethal Screen
- Synthetic Genetic Array Analysis in Saccharomyces cerevisiae
- Two-Dimensional Agarose Gel Analysis of DNA Replication Intermediates
- Chromatin Assembly in a Crude Fraction From Yeast Cells
- Chromatin Immunoprecipitation to Study Protein-DNA Interactions in Budding Yeast
- Isolation of Yeast Nuclei and Micrococcal Nuclease Mapping of Nucleosome Positioning
- Study of Transcriptional Regulation Using a Reporter Gene Assay
- Assessing Telomeric Phenotypes
- Controlled Expression of Recombinant Genes and Preparation of Cell-Free Extracts in Yeast
- Production of Heterologous Proteins in Yeast With the Aid of the Hsp150? Carrier
- Yeast Two-Hybrid System Screening
- Import of Precursor Proteins Into Isolated Yeast Mitochondria.