Neural Stem Cells Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Weiner, Leslie P. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2008.
Edición:2nd ed. 2008.
Colección:Methods in Molecular Biology, 438
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-59745-133-8
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245 1 0 |a Neural Stem Cells  |b Methods and Protocols /  |c edited by Leslie P. Weiner. 
250 |a 2nd ed. 2008. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2008. 
300 |a XVI, 406 p. 53 illus., 2 illus. in color.  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
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490 1 |a Methods in Molecular Biology,  |v 438 
505 0 |a Definitions and Criteria for Stem Cells -- Neural Stem Cells and the Future Treatment of Neurological Diseases: Raising the Standard -- Isolation and Culture of NSCs -- Neural Differentiation of Human Embryonic Stem Cells -- Generating Neurons from Stem Cells -- Isolation of Neural Stem and Precursor Cells from Rodent Tissue -- Adult Rodent Spinal Cord-Derived Neural Stem Cells: Isolation and Characterization -- Adult Neural Stem Cells -- Olfactory Ensheathing Cells: Isolation and Culture from the Neonatal Olfactory Bulb -- Culturing Olfactory Ensheathing Cells from the Mouse Olfactory Epithelium -- Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentiation Property -- Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood -- Production of Neurospheres from CNS Tissue -- Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells -- In Vitro Studies: A. Cellular Techniques -- Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures -- Telomere Neurobiology -- Chromosomal Mosaicism in Neural Stem Cells -- In Vitro Assays for Neural Stem Cell Differentiation: Induction of Dopaminergic Phenotype -- In Vitro Migration Assays of Neural Stem Cells -- Measuring Apoptosis in Neural Stem Cells -- Markers of Adult Neural Stem Cells -- In Vitro Studies: B. Molecular Techniques -- Differential Gene Expression in ES-Derived Neural Stem Cells by Using RT-PCR -- Microfluidic Devices for High-Throughput Gene Expression Profiling of Single hESC-Derived Neural Stem Cells -- Electroporation Strategies for Genetic Manipulation and Cell Labeling -- Novel and Immortalization-Based Protocols for the Generation of Neural CNS Stem Cell Lines for Gene Therapy Approaches -- Studies for Transplantation -- Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo -- Immunocytochemical Analysis of Neuronal Differentiation -- Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold -- Labeling Stem Cells In Vitro for Identification of their Differentiated Phenotypes after Grafting into the CNS -- Optimizing Stem Cell Grafting into the CNS -- Routes of Stem Cell Administration in the Adult Rodent. 
650 0 |a Anthropology. 
650 0 |a Neurosciences. 
650 0 |a Cytology. 
650 0 |a Neurobiology. 
650 1 4 |a Anthropology. 
650 2 4 |a Neurosciences. 
650 2 4 |a Cell Biology. 
650 2 4 |a Neurobiology. 
700 1 |a Weiner, Leslie P.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
856 4 0 |u https://doi.org/10.1007/978-1-59745-133-8