Neural Stem Cells Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Weiner, Leslie P. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2008.
Edición:2nd ed. 2008.
Colección:Methods in Molecular Biology, 438
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-59745-133-8
Tabla de Contenidos:
  • Definitions and Criteria for Stem Cells
  • Neural Stem Cells and the Future Treatment of Neurological Diseases: Raising the Standard
  • Isolation and Culture of NSCs
  • Neural Differentiation of Human Embryonic Stem Cells
  • Generating Neurons from Stem Cells
  • Isolation of Neural Stem and Precursor Cells from Rodent Tissue
  • Adult Rodent Spinal Cord-Derived Neural Stem Cells: Isolation and Characterization
  • Adult Neural Stem Cells
  • Olfactory Ensheathing Cells: Isolation and Culture from the Neonatal Olfactory Bulb
  • Culturing Olfactory Ensheathing Cells from the Mouse Olfactory Epithelium
  • Production and Characterization of Immortal Human Neural Stem Cell Line with Multipotent Differentiation Property
  • Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood
  • Production of Neurospheres from CNS Tissue
  • Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells
  • In Vitro Studies: A. Cellular Techniques
  • Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures
  • Telomere Neurobiology
  • Chromosomal Mosaicism in Neural Stem Cells
  • In Vitro Assays for Neural Stem Cell Differentiation: Induction of Dopaminergic Phenotype
  • In Vitro Migration Assays of Neural Stem Cells
  • Measuring Apoptosis in Neural Stem Cells
  • Markers of Adult Neural Stem Cells
  • In Vitro Studies: B. Molecular Techniques
  • Differential Gene Expression in ES-Derived Neural Stem Cells by Using RT-PCR
  • Microfluidic Devices for High-Throughput Gene Expression Profiling of Single hESC-Derived Neural Stem Cells
  • Electroporation Strategies for Genetic Manipulation and Cell Labeling
  • Novel and Immortalization-Based Protocols for the Generation of Neural CNS Stem Cell Lines for Gene Therapy Approaches
  • Studies for Transplantation
  • Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo
  • Immunocytochemical Analysis of Neuronal Differentiation
  • Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold
  • Labeling Stem Cells In Vitro for Identification of their Differentiated Phenotypes after Grafting into the CNS
  • Optimizing Stem Cell Grafting into the CNS
  • Routes of Stem Cell Administration in the Adult Rodent.