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02879nam a22003135i 4500 |
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978-1-59745-158-1 |
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20190618101645.0 |
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cr nn 008mamaa |
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100301s2006 xxu| s |||| 0|eng d |
| 020 |
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|a 9781597451581
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| 024 |
7 |
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|a 10.1385/1597451584
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a YAC Protocols
|c edited by Alasdair MacKenzie.
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| 250 |
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|a 2nd ed. 2006.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2006.
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| 300 |
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|a XVI, 204 p. 49 illus.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 349
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| 505 |
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|a Markers, Selection, and Media in Yeast Artificial Chromosome Cloning -- Construction of Yeast Artificial Chromosome Libraries From Pathogens and Nonmodel Organisms -- Analysis and Screening of Yeast Artificial Chromosome Libraries by Filter Hybridization -- Analysis of Yeast Artificial Chromosome DNA by Restriction Digestion, Southern Blotting Nucleic Acid Hybridization, and Polymerase Chain Reaction -- Postgenomic Bioinformatic Analysis of Yeast Artificial Chromosome Sequence -- Transformation of Yeast Using Bioactive Beads With Surface-Immobilized Yeast Artificial Chromosomes -- Modification and Amplification of Yeast Artificial Chromosomes -- Modifying Yeast Artificial Chromosomes to Generate Cre/LoxP and FLP/FRT Site-Specific Deletions and Inversions -- Selective Isolation of Large Chromosomal Regions by Transformation-Associated Recombination Cloning for Structural and Functional Analysis of Mammalian Genomes -- A Polymerase Chain Reaction-Mediated Yeast Artificial Chromosome-Splitting Technology for Generating Targeted Yeast Artificial Chromosomes Subclones -- Genomic Reconstruction by Serial Mitotic Recombination of Yeast Artificial Chromosomes -- Targeting Vector Construction by Yeast Artificial Chromosome Modification -- Production of Yeast Artificial Chromosome Transgenic Mice by Pronuclear Injection of One-Cell Embryos -- Generation of Yeast Artificial Chromosome Transgenic Mice by Intracytoplasmic Sperm Injection -- Establishment of Cell Lines That Exhibit Correct Ontogenic Stage-Specific Gene Expression Profiles From Tissues of Yeast Artificial Chromosome Transgenic Mice Using Chemically Induced Growth Signals -- Application of Yeast Artificial Chromosomes in Fluorescence In Situ Hybridization -- Contribution of Yeast Artificial Chromosome-Based Physical Maps to the Final Assembly of the Trypanosoma cruzi Genome.
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| 650 |
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|a Biochemistry.
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| 650 |
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|a Human genetics.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Human Genetics.
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| 700 |
1 |
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|a MacKenzie, Alasdair.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1385/1597451584
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