YAC Protocols
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2006.
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| Edición: | 2nd ed. 2006. |
| Colección: | Methods in Molecular Biology,
349 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/1597451584 |
Tabla de Contenidos:
- Markers, Selection, and Media in Yeast Artificial Chromosome Cloning
- Construction of Yeast Artificial Chromosome Libraries From Pathogens and Nonmodel Organisms
- Analysis and Screening of Yeast Artificial Chromosome Libraries by Filter Hybridization
- Analysis of Yeast Artificial Chromosome DNA by Restriction Digestion, Southern Blotting Nucleic Acid Hybridization, and Polymerase Chain Reaction
- Postgenomic Bioinformatic Analysis of Yeast Artificial Chromosome Sequence
- Transformation of Yeast Using Bioactive Beads With Surface-Immobilized Yeast Artificial Chromosomes
- Modification and Amplification of Yeast Artificial Chromosomes
- Modifying Yeast Artificial Chromosomes to Generate Cre/LoxP and FLP/FRT Site-Specific Deletions and Inversions
- Selective Isolation of Large Chromosomal Regions by Transformation-Associated Recombination Cloning for Structural and Functional Analysis of Mammalian Genomes
- A Polymerase Chain Reaction-Mediated Yeast Artificial Chromosome-Splitting Technology for Generating Targeted Yeast Artificial Chromosomes Subclones
- Genomic Reconstruction by Serial Mitotic Recombination of Yeast Artificial Chromosomes
- Targeting Vector Construction by Yeast Artificial Chromosome Modification
- Production of Yeast Artificial Chromosome Transgenic Mice by Pronuclear Injection of One-Cell Embryos
- Generation of Yeast Artificial Chromosome Transgenic Mice by Intracytoplasmic Sperm Injection
- Establishment of Cell Lines That Exhibit Correct Ontogenic Stage-Specific Gene Expression Profiles From Tissues of Yeast Artificial Chromosome Transgenic Mice Using Chemically Induced Growth Signals
- Application of Yeast Artificial Chromosomes in Fluorescence In Situ Hybridization
- Contribution of Yeast Artificial Chromosome-Based Physical Maps to the Final Assembly of the Trypanosoma cruzi Genome.