Prion Protein Protocols

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Hill, Andrew F. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2008.
Edición:1st ed. 2008.
Colección:Methods in Molecular Biology, 459
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-59745-234-2
Tabla de Contenidos:
  • Cell Culture Models to Unravel Prion Protein Function and Aberrancies in Prion Diseases
  • Investigation of PrPC Metabolism and Function in Live Cells
  • Immunodetection of PrPSc Using Western and Slot Blotting Techniques
  • Assaying Prions in Cell Culture
  • Generation of Cell Lines Propagating Infectious Prions and the Isolation and Characterization of Cell-derived Exosomes
  • Neurotoxicity of Prion Peptides on Cultured Cerebellar Neurons
  • Understanding the Nature of Prion Diseases Using Cell-free Assays
  • Methods for Conversion of Prion Protein into Amyloid Fibrils
  • Amplification of Purified Prions In Vitro
  • Expression and Purification of Full-Length Recombinant PrP of High Purity
  • Analysis of PrP Conformation Using Circular Dichroism
  • Effect of Copper on the De Novo Generation of Prion Protein Expressed in Pichia pastoris
  • Biophysical Investigations of the Prion Protein Using Electron Paramagnetic Resonance
  • Molecular Diagnosis of Human Prion Disease
  • Analysis of Endogenous PrPC Processing in Neuronal and Non-neuronal Cell Lines
  • Molecular Typing of PrPres in Human Sporadic CJD Brain Tissue
  • Transgenic Mouse Models of Prion Diseases
  • Quantitative Bioassay of Surface-bound Prion Infectivity.