|
|
|
|
| LEADER |
03309nam a22003135i 4500 |
| 001 |
978-1-59745-267-0 |
| 005 |
20191026202221.0 |
| 007 |
cr nn 008mamaa |
| 008 |
100301s2007 xxu| s |||| 0|eng d |
| 020 |
|
|
|a 9781597452670
|
| 024 |
7 |
|
|a 10.1385/159745267X
|2 doi
|
| 040 |
|
|
|a Sistema de Bibliotecas del Tecnológico de Costa Rica
|
| 245 |
1 |
0 |
|a Protein Phosphatase Protocols
|c edited by Greg Moorhead.
|
| 250 |
|
|
|a 1st ed. 2007.
|
| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2007.
|
| 300 |
|
|
|a XV, 387 p.
|b online resource.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a computer
|b c
|2 rdamedia
|
| 338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
| 490 |
1 |
|
|a Methods in Molecular Biology,
|v 365
|
| 505 |
0 |
|
|a Analysis of Protein Phosphatases -- A Brief Introduction to the Protein Phosphatase Families -- Small-Molecule Inhibitors of Ser/Thr Protein Phosphatases -- Synthesis and Use of the Protein Phosphatase Affinity Matrices Microcystin-Sepharose and Microcystin-Biotin-Sepharose -- Utilizing Protein Phosphatase Inhibitors to Define PP2A as a Regulator of Ataxia-Telangiectasia Mutated -- An Automated Fluorescence-Based Method for Continuous Assay of PP2A Activity -- An In Vivo Assay to Quantify Stable Protein Phosphatase 2A (PP2A) Heterotrimeric Species -- Mutagenesis and Expression of the Scaffolding A? and A? Subunits of PP2A -- Isolation and Characterization of PP2A Holoenzymes Containing FLAG-Tagged B Subunits -- Purification of PP2A Holoenzymes by Sequential Immunoprecipitation with Anti-Peptide Antibodies -- Purification of PP2Ac from Bovine Heart -- Visualization of Intracellular PP1 Targeting Through Transiently and Stably Expressed Fluorescent Protein Fusions -- Yeast Two-Hybrid Screens to Identify Drosophila PP1-Binding Proteins -- Identification of Cellular Protein Phosphatase-1 Regulators -- Assay for Three-Way Interaction of Protein Phosphatase-1 (Glc7) With Regulatory Subunits Plus Phosphatase Inhibitor-2 -- Phosphorylation of the Protein Phosphatase Type 1 Inhibitor Protein CPI-17 by Protein Kinase C -- Purification of Smooth Muscle Myosin Phosphatase Using a Thiophosphorylated Myosin Light-Chain-Affinity Resin -- Proteins Interacting With Saccharomyces cerevisiae Type 1 Protein Phosphatase Catalytic Subunit Identified by Single-Step Affinity Purification and Mass Spectrometry -- Expression of Protein Histidine Phosphatase in Escherichia coli, Purification, and Determination of Enzyme Activity -- The Use of RNA Interference to Analyze Protein Phosphatase Function in Mammalian Cells -- Recognition of a PP2C Interaction Motif in Several Plant Protein Kinases -- Use of Yeast Genetic Tools to Define Biological Roles of Novel Protein Phosphatases -- Targeting of PP2C in Budding Yeast -- Phosphatase Targets in TOR Signaling -- Functional Characterization of Small CTD Phosphatases -- Genome-Scale Discovery and Characterization of Class-Specific Protein Sequences -- Yeast Substrate-Trapping System for Isolating Substrates of Protein Tyrosine Phosphatases.
|
| 650 |
|
0 |
|a Biochemistry.
|
| 650 |
|
0 |
|a Cell biology.
|
| 650 |
1 |
4 |
|a Biochemistry, general.
|
| 650 |
2 |
4 |
|a Cell Biology.
|
| 700 |
1 |
|
|a Moorhead, Greg.
|e editor.
|
| 710 |
2 |
|
|a SpringerLink (Online service)
|
| 773 |
0 |
|
|t Springer eBooks
|
| 856 |
4 |
0 |
|u https://doi.org/10.1385/159745267X
|