Protein Phosphatase Protocols
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2007.
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| Edición: | 1st ed. 2007. |
| Colección: | Methods in Molecular Biology,
365 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1385/159745267X |
Tabla de Contenidos:
- Analysis of Protein Phosphatases
- A Brief Introduction to the Protein Phosphatase Families
- Small-Molecule Inhibitors of Ser/Thr Protein Phosphatases
- Synthesis and Use of the Protein Phosphatase Affinity Matrices Microcystin-Sepharose and Microcystin-Biotin-Sepharose
- Utilizing Protein Phosphatase Inhibitors to Define PP2A as a Regulator of Ataxia-Telangiectasia Mutated
- An Automated Fluorescence-Based Method for Continuous Assay of PP2A Activity
- An In Vivo Assay to Quantify Stable Protein Phosphatase 2A (PP2A) Heterotrimeric Species
- Mutagenesis and Expression of the Scaffolding A? and A? Subunits of PP2A
- Isolation and Characterization of PP2A Holoenzymes Containing FLAG-Tagged B Subunits
- Purification of PP2A Holoenzymes by Sequential Immunoprecipitation with Anti-Peptide Antibodies
- Purification of PP2Ac from Bovine Heart
- Visualization of Intracellular PP1 Targeting Through Transiently and Stably Expressed Fluorescent Protein Fusions
- Yeast Two-Hybrid Screens to Identify Drosophila PP1-Binding Proteins
- Identification of Cellular Protein Phosphatase-1 Regulators
- Assay for Three-Way Interaction of Protein Phosphatase-1 (Glc7) With Regulatory Subunits Plus Phosphatase Inhibitor-2
- Phosphorylation of the Protein Phosphatase Type 1 Inhibitor Protein CPI-17 by Protein Kinase C
- Purification of Smooth Muscle Myosin Phosphatase Using a Thiophosphorylated Myosin Light-Chain-Affinity Resin
- Proteins Interacting With Saccharomyces cerevisiae Type 1 Protein Phosphatase Catalytic Subunit Identified by Single-Step Affinity Purification and Mass Spectrometry
- Expression of Protein Histidine Phosphatase in Escherichia coli, Purification, and Determination of Enzyme Activity
- The Use of RNA Interference to Analyze Protein Phosphatase Function in Mammalian Cells
- Recognition of a PP2C Interaction Motif in Several Plant Protein Kinases
- Use of Yeast Genetic Tools to Define Biological Roles of Novel Protein Phosphatases
- Targeting of PP2C in Budding Yeast
- Phosphatase Targets in TOR Signaling
- Functional Characterization of Small CTD Phosphatases
- Genome-Scale Discovery and Characterization of Class-Specific Protein Sequences
- Yeast Substrate-Trapping System for Isolating Substrates of Protein Tyrosine Phosphatases.