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03154nam a22003735i 4500 |
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978-1-59745-281-6 |
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20191026222128.0 |
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cr nn 008mamaa |
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110414s2009 xxu| s |||| 0|eng d |
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|a 9781597452816
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|a 10.1007/978-1-59745-281-6
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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|a Two-Dimensional Electrophoresis Protocols
|c edited by David Sheehan, Raymond Tyther.
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| 250 |
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|a 1st ed. 2009.
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| 260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2009.
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| 300 |
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|a XIV, 546 p. 100 illus., 1 illus. in color.
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
|v 519
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|a Two-Dimensional Electrophoresis: An Overview -- Solubilization of Proteins in 2DE: An Outline -- Selection of pH Ranges in 2DE -- Difficult Proteins -- Organelle Proteomics -- Applications of Chemical Tagging Approaches in Combination with 2DE and Mass Spectrometry -- Immunoblotting 2DE Membranes -- Troubleshooting Image Analysis in 2DE -- Analysis of Bacterial Proteins by 2DE -- Proteomic Analysis of Caenorhabditis elegans -- Protein Extraction for 2DE -- Analysis of Proteins from Marine Molluscs -- Preparation and Analysis of Plant and Plastid Proteomes by 2DE. -- High-Resolution 2DE -- Blue Native-Gel Electrophoresis Proteomics -- 2DE for Proteome Analysis of Human Metaphase Chromosomes -- Microsomal Proteomics -- Prefractionation Using Microscale Solution IEF -- Diagonal Electrophoresis for Detection of Protein Disulphide Bridges -- High-Resolution Large-Gel 2DE -- Silver Staining of Proteins in 2DE Gels -- Detection of 4-Hydroxy-2-Nonenal- and 3-Nitrotyrosine-Modified Proteins Using a Proteomics Approach -- Proteomic Detection of Oxidized and Reduced Thiol Proteins in Cultured Cells -- Detection of Ubiquitination in 2DE -- Phosphoproteome Analysis by In-Gel Isoelectric Focusing and Tandem Mass Spectrometry -- Detection of Protein Glutathionylation -- Activity-Based Protein Profiling of Protein Tyrosine Phosphatases -- Active Protease Mapping in 2DE Gels -- Two-Dimensional Difference Gel Electrophoresis -- Protein Expression Profiling -- C-Terminal Sequence Analysis of 2DE-Separated Proteins -- Shotgun Protein Analysis by Liquid Chromatography-Tandem Mass Spectrometry -- de Novo Sequence Analysis of N-Terminal Sulfonated Peptides After in-Gel Guanidination -- Tryptic Digestion of In-Gel Proteins for Mass Spectrometry Analysis -- Database Interrogation Algorithms for Identification of Proteins in Proteomic Separations -- Creating 2DE Databases for the World Wide Web.
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| 650 |
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|a Biochemistry.
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| 650 |
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|a Proteins .
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| 650 |
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|a Proteomics.
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| 650 |
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|a Electrophoresis.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Protein Science.
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| 650 |
2 |
4 |
|a Proteomics.
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| 650 |
2 |
4 |
|a Electrophoresis.
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| 700 |
1 |
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|a Sheehan, David.
|e editor.
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| 700 |
1 |
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|a Tyther, Raymond.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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| 856 |
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|u https://doi.org/10.1007/978-1-59745-281-6
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