Two-Dimensional Electrophoresis Protocols
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2009.
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| Edición: | 1st ed. 2009. |
| Colección: | Methods in Molecular Biology,
519 |
| Materias: | |
| Acceso en línea: | https://doi.org/10.1007/978-1-59745-281-6 |
Tabla de Contenidos:
- Two-Dimensional Electrophoresis: An Overview
- Solubilization of Proteins in 2DE: An Outline
- Selection of pH Ranges in 2DE
- Difficult Proteins
- Organelle Proteomics
- Applications of Chemical Tagging Approaches in Combination with 2DE and Mass Spectrometry
- Immunoblotting 2DE Membranes
- Troubleshooting Image Analysis in 2DE
- Analysis of Bacterial Proteins by 2DE
- Proteomic Analysis of Caenorhabditis elegans
- Protein Extraction for 2DE
- Analysis of Proteins from Marine Molluscs
- Preparation and Analysis of Plant and Plastid Proteomes by 2DE.
- High-Resolution 2DE
- Blue Native-Gel Electrophoresis Proteomics
- 2DE for Proteome Analysis of Human Metaphase Chromosomes
- Microsomal Proteomics
- Prefractionation Using Microscale Solution IEF
- Diagonal Electrophoresis for Detection of Protein Disulphide Bridges
- High-Resolution Large-Gel 2DE
- Silver Staining of Proteins in 2DE Gels
- Detection of 4-Hydroxy-2-Nonenal- and 3-Nitrotyrosine-Modified Proteins Using a Proteomics Approach
- Proteomic Detection of Oxidized and Reduced Thiol Proteins in Cultured Cells
- Detection of Ubiquitination in 2DE
- Phosphoproteome Analysis by In-Gel Isoelectric Focusing and Tandem Mass Spectrometry
- Detection of Protein Glutathionylation
- Activity-Based Protein Profiling of Protein Tyrosine Phosphatases
- Active Protease Mapping in 2DE Gels
- Two-Dimensional Difference Gel Electrophoresis
- Protein Expression Profiling
- C-Terminal Sequence Analysis of 2DE-Separated Proteins
- Shotgun Protein Analysis by Liquid Chromatography-Tandem Mass Spectrometry
- de Novo Sequence Analysis of N-Terminal Sulfonated Peptides After in-Gel Guanidination
- Tryptic Digestion of In-Gel Proteins for Mass Spectrometry Analysis
- Database Interrogation Algorithms for Identification of Proteins in Proteomic Separations
- Creating 2DE Databases for the World Wide Web.