Leukemia Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: So, Chi Wai Eric. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:1st ed. 2009.
Colección:Methods in Molecular Biology, 538
Materias:
Acceso en línea:https://doi.org/10.1007/978-1-59745-418-6
Tabla de Contenidos:
  • An Overview: From Discovery of Candidate Mutations to Disease Modeling and Transformation Mechanisms of Acute Leukemia
  • Backtracking of Leukemic Clones to Birth
  • Fluorescence In Situ Hybridization (FISH) as a Tool for the Detection of Significant Chromosomal Abnormalities in Childhood Leukaemia
  • Specialized Fluorescence In Situ Hybridization (FISH) Techniques for Leukaemia Research
  • LDI-PCR: Identification of Known and Unknown Gene Fusions of the Human MLL Gene
  • Panhandle PCR Approaches to Cloning MLL Genomic Breakpoint Junctions and Fusion Transcript Sequences
  • MRD Detection in Acute Lymphoblastic Leukemia Patients Using Ig/TCR Gene Rearrangements as Targets for Real-Time Quantitative PCR
  • Array-Based Comparative Genomic Hybridization as a Tool for Analyzing the Leukemia Genome
  • Application of SNP Genotype Arrays to Determine Somatic Changes in Cancer
  • Retroviral/Lentiviral Transduction and Transformation Assay
  • Gene Expression Profiling of Leukemia Stem Cells
  • Humanized Model to Study Leukemic Stem Cells
  • Model Systems for Examining Effects of Leukemia Associated Oncogenes in Primary Human CD34+ Cells via Retroviral Transduction
  • Ex Vivo Assays to Study Self-Renewal and Long-Term Expansion of Genetically Modified Primary Human Acute Myeloid Leukemia Stem Cells
  • Identification and Characterization of Hematopoietic Stem and Progenitor Cell Populations in Mouse Bone Marrow by Flow Cytometry
  • In Vitro Differentiation of Embryonic Stem Cells as a Model of Early Hematopoietic Development
  • Analysis of the Mouse Placenta as a Hematopoietic Stem Cell Niche
  • Identification of Protein Interaction Partners by the Yeast Two-Hybrid System
  • Complete Array of HOX Gene Expression by RQ-PCR
  • Genome-Wide Determination of DNA Methylation by Hpa II Tiny Fragment Enrichment by Ligation-Mediated PCR (HELP) for the Study of Acute Leukemias
  • Chromatin Immunoprecipitation (ChIP) for Analysis of Histone Modifications and Chromatin-Associated Proteins.